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Spontaneous programmed cell death of peripheral blood mononuclear cells from HIV-infected persons is decreased with interleukin-15
DC Field | Value | Language |
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dc.contributor.author | 김준명 | - |
dc.contributor.author | 송영구 | - |
dc.date.accessioned | 2019-11-11T05:27:51Z | - |
dc.date.available | 2019-11-11T05:27:51Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171899 | - |
dc.description.abstract | Interleukin 15 (IL-15) is an important regulatory cytokine in cellular immunity. In vitro replacement of IL-15 has been shown to enhance immunity in Human immunodeficiency virus type 1 (HIV-1) infected lymphocytes. We evaluated the effect of IL-15 on the survival of peripheral blood mononuclear cells of HIV patients by examining in vitro lymphocyte apoptosis, and correlated the process with Bcl-2 and Fas gene regulation. Peripheral blood mononuclear cells (PBMC) from 21 HIV-infected adults and 24 HIV-seronegative healthy individuals were isolated and cultured to determine the effect of escalating doses of IL-15 (0, 1, 10, 100, 1000 ng/mL) on apoptosis. Lymphocyte proliferation assay with (3H) TdR was measured and Bcl-2 and Fas gene regulation was observed. The results were as follows: 1) IL-15 reduced culture induced lymphocyte apoptosis in HIV patients in a dose dependent manner, and reached a plateau level at a concentration of 100 ng/ml; 2) IL-15 significantly reduced the level of apoptosis after 3 days (14%) and 5 days (15%) of culture in HIV patients, while no difference was observed in HIV (-) donors; 3) The percentage of viable cells among the total number of lymphocytes was significantly enhanced by 25% in HIV patients with IL-15; 4) Bcl-2 expression was decreased in HIV patients (53.9 +/- 12.3%) compared to HIV (-) donors (93.0 +/- 3.7%), and IL-15 increased Bcl-2 expression by 21.2 +/- 5.2% in HIV patients; 5) Fas expression was increased in HIV patients (70.2 +/- 4.6%) compared to HIV (-) donors (32.4 +/- 4.3%), and IL-15 increased Fas expression by 8.4 +/- 1.2% in HIV (-) donors. Our findings indicate that IL-15 may influence immunologic abnormalities in HIV infection, particularly its ability to prevent apoptosis of lymphocytes by suppressing the down-modulation of Bcl-2. This may provide an experimental basis for IL-15 immunotherapy | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | Yonsei Medical Journal | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Gene Expression Regulation/physiology | - |
dc.subject.MESH | Genes, bcl-2/genetics | - |
dc.subject.MESH | HIV Infections/blood* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-15/pharmacology* | - |
dc.subject.MESH | Monocytes/drug effects* | - |
dc.subject.MESH | fas Receptor/genetics | - |
dc.title | Spontaneous programmed cell death of peripheral blood mononuclear cells from HIV-infected persons is decreased with interleukin-15 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Kyung Hee Chang | - |
dc.contributor.googleauthor | June Myung Kim | - |
dc.contributor.googleauthor | Hyo Youl Kim | - |
dc.contributor.googleauthor | Young Goo Song | - |
dc.contributor.googleauthor | Young Hwa Choi | - |
dc.contributor.googleauthor | Yoon soo Park | - |
dc.contributor.googleauthor | Jung Ho Cho | - |
dc.contributor.googleauthor | Sung Kwan Hong | - |
dc.identifier.doi | 10.3349/ymj.2000.41.1.112 | - |
dc.contributor.localId | A00953 | - |
dc.contributor.localId | A02037 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 10731928 | - |
dc.subject.keyword | Interleukin 15 | - |
dc.subject.keyword | lymphocyte | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | Human Immunodeficiency Virus | - |
dc.contributor.alternativeName | Kim, June Myung | - |
dc.contributor.affiliatedAuthor | 김준명 | - |
dc.contributor.affiliatedAuthor | 송영구 | - |
dc.citation.volume | 41 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 112 | - |
dc.citation.endPage | 118 | - |
dc.identifier.bibliographicCitation | Yonsei Medical Journal, Vol.41(1) : 112-118, 2000 | - |
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