Cited 14 times in
Creation and Initial Characterization of Isogenic Helicobacter pylori CagA EPIYA Variants Reveals Differential Activation of Host Cell Signaling Pathways
DC Field | Value | Language |
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dc.contributor.author | 장성일 | - |
dc.contributor.author | 차정헌 | - |
dc.date.accessioned | 2018-07-20T08:14:11Z | - |
dc.date.available | 2018-07-20T08:14:11Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160998 | - |
dc.description.abstract | The polymorphic CagA toxin is associated with Helicobacter pylori-induced disease. Previous data generated using non-isogenic strains and transfection models suggest that variation surrounding the C-terminal Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs as well as the number of EPIYA motifs influence disease outcome. To investigate potential CagA-mediated effects on host cell signaling, we constructed and characterized a large panel of isogenic H. pylori strains that differ primarily in the CagA EPIYA region. The number of EPIYA-C motifs or the presence of an EPIYA-D motif impacted early changes in host cell elongation; however, the degree of elongation was comparable across all strains at later time points. In contrast, the strain carrying the EPIYA-D motif induced more IL-8 secretion than any other EPIYA type, and a single EPIYA-C motif induced comparable IL-8 secretion as isolates carrying multiple EPIYA-C alleles. Similar levels of ERK1/2 activation were induced by all strains carrying a functional CagA allele. Together, our data suggest that polymorphism in the CagA C-terminus is responsible for differential alterations in some, but not all, host cell signaling pathways. Notably, our results differ from non-isogenic strain studies, thus highlighting the importance of using isogenic strains to study the role of CagA toxin polymorphism in gastric cancer development. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Creation and Initial Characterization of Isogenic Helicobacter pylori CagA EPIYA Variants Reveals Differential Activation of Host Cell Signaling Pathways | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Oral Biology | - |
dc.contributor.googleauthor | Dacie R. Bridge | - |
dc.contributor.googleauthor | Faith C. Blum | - |
dc.contributor.googleauthor | Sungil Jang | - |
dc.contributor.googleauthor | Jinmoon Kim | - |
dc.contributor.googleauthor | Jeong-Heon Cha | - |
dc.contributor.googleauthor | D. Scott Merrell | - |
dc.identifier.doi | 10.1038/s41598-017-11382-y | - |
dc.contributor.localId | A03440 | - |
dc.contributor.localId | A04007 | - |
dc.relation.journalcode | J02646 | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.pmid | 28887533 | - |
dc.contributor.alternativeName | Jang, Sung Il | - |
dc.contributor.alternativeName | Cha, Jung Heon | - |
dc.contributor.affiliatedAuthor | Jang, Sungil | - |
dc.contributor.affiliatedAuthor | Cha, Jung Heon | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 11057 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, Vol.7(1) : 11057, 2017 | - |
dc.identifier.rimsid | 60890 | - |
dc.type.rims | ART | - |
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