Cited 3 times in
Genetic and biochemical characterisation of CTX-M-37 extended-spectrum β-lactamase from an Enterobacter cloacae clinical isolate from Mongolia
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.contributor.author | 이경원 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2018-07-20T08:06:08Z | - |
dc.date.available | 2018-07-20T08:06:08Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2213-7165 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160838 | - |
dc.description.abstract | OBJECTIVES: The aims of this study were to determine the resistance level of a blaCTX-M-37-carrying Enterobacter cloacae isolate from Mongolia, to analyse kinetic parameters of the purified enzyme and to compare the genetic environment of the gene. METHODS: Minimum inhibitory concentrations (MICs) were determined using the Clinical and Laboratory Standards Institute (CLSI) agar dilution method. Purified CTX-M-37 enzyme was used to determined kinetic parameters. The genetic environment of the blaCTX-M-37 gene in E. cloacae was compared with a Kluyvera cryocrescens isolate. RESULTS: The E. cloacae isolate showed relatively low-level resistance to cefotaxime (MIC=16mg/L) compared with a CTX-M-3-producing strain (MIC=256mg/L), and CTX-M-37 had a lower kcat/Km value for cefotaxime (2.0μM-1s-1) compared with CTX-M-3 (3.5μM-1s-1), possibly due to Asn114Asp substitution. The blaCTX-M-37 gene in the E. cloacae isolate was carried on a conjugative plasmid and was associated with an ISEcp1 element containing the -35 and -10 putative promoter sequences TTGAAA and TACAAT, respectively, unlike in the K. cryocrescens isolate. CONCLUSIONS: The CTX-M-37-producing E. cloacae isolate showed relatively low-level resistance to cefotaxime and the purified enzyme had lower kinetic parameters as the result of Asn114Asp substitution. Presence of an ISEcp1 element and putative promoters upstream of the blaCTX-M-37 gene in E. cloacae, but not in the K. cryocrescens isolate, indicated their roles in mobilisation and expression of the gene. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Ltd. | - |
dc.relation.isPartOf | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Genetic and biochemical characterisation of CTX-M-37 extended-spectrum β-lactamase from an Enterobacter cloacae clinical isolate from Mongolia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Laboratory Medicine | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Khosbayar Tulgaa | - |
dc.contributor.googleauthor | Jean-Denis Docquier | - |
dc.contributor.googleauthor | Gian Maria Rossolini | - |
dc.contributor.googleauthor | Yunsop Chong | - |
dc.identifier.doi | 10.1016/j.jgar.2017.03.007 | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J01427 | - |
dc.identifier.eissn | 2213-7173 | - |
dc.identifier.pmid | 28587869 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S2213716517300723 | - |
dc.subject.keyword | CTX-M-37 | - |
dc.subject.keyword | Enterobacter cloacae | - |
dc.subject.keyword | Extended-spectrum β-lactamase | - |
dc.subject.keyword | Genetic environment | - |
dc.subject.keyword | Kinetic parameters | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.citation.volume | 10 | - |
dc.citation.startPage | 3 | - |
dc.citation.endPage | 7 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, Vol.10 : 3-7, 2017 | - |
dc.identifier.rimsid | 60722 | - |
dc.type.rims | ART | - |
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