Cited 12 times in
Intravitreal itraconazole inhibits laser-induced choroidal neovascularization in rats
DC Field | Value | Language |
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dc.contributor.author | 고형준 | - |
dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 양우익 | - |
dc.contributor.author | 이성철 | - |
dc.date.accessioned | 2018-07-20T07:41:26Z | - |
dc.date.available | 2018-07-20T07:41:26Z | - |
dc.date.issued | 2017 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/160424 | - |
dc.description.abstract | Choroidal neovascularization (CNV) is a major cause of severe visual loss in patients with age-related macular degeneration (AMD). Recently, itraconazole has shown potent and dose-dependent inhibition of tumor-associated angiogenesis. We evaluated the anti-angiogenic effect of itraconazole in a rat model of laser-induced CNV. After laser photocoagulation in each eye to cause CNV, right eyes were administered intravitreal injections of itraconazole; left eyes received balanced salt solution (BSS) as controls. On day 14 after laser induction, fluorescein angiography (FA) was used to assess abnormal vascular leakage. Flattened retinal pigment epithelium (RPE)-choroid tissue complex was stained with Alexa Fluor 594-conjugated isolectin B4 to measure the CNV area and volume. Vascular endothelial growth factor receptor 2 (VEGFR2) mRNA and protein expression was determined 1, 4, 7, and 14 days after intravitreal injection by quantitative RT-PCR or Western blot. VEGF levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Intravitreal itraconazole significantly reduced leakage from CNV as assessed by FA and CNV area and volume on flat mounts compared with intravitreal BSS (p = 0.002 for CNV leakage, p<0.001 for CNV area and volume). Quantitative RT-PCR showed significantly lower expression of VEGFR2 mRNA in the RPE-choroid complexes of itraconazole-injected eyes than those of BSS-injected eyes on days 7 and 14 (p = 0.003 and p = 0.006). Western blots indicated that VEGFR2 was downregulated after itraconazole treatment. ELISA showed a significant difference in VEGF level between itraconazole-injected and BSS-injected eyes on days 7 and 14 (p = 0.04 and p = 0.001). Our study demonstrated that intravitreal itraconazole significantly inhibited the development of laser-induced CNV in rats. Itraconazole had anti-angiogenic activity along with the reduction of VEGFR2 and VEGF levels. Itraconazole may prove beneficial for treating CNV as an alternative or adjunct to other therapies. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Public Library of Science | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Intravitreal itraconazole inhibits laser-induced choroidal neovascularization in rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Ophthalmology | - |
dc.contributor.googleauthor | Jeong Hun Bae | - |
dc.contributor.googleauthor | Ah Reum Hwang | - |
dc.contributor.googleauthor | Chan Yun Kim | - |
dc.contributor.googleauthor | Hyeong Gon Yu | - |
dc.contributor.googleauthor | Hyoung Jun Koh | - |
dc.contributor.googleauthor | Woo Ick Yang | - |
dc.contributor.googleauthor | Hae Ran Chang | - |
dc.contributor.googleauthor | Sung Chul Lee | - |
dc.identifier.doi | 10.1371/journal.pone.0180482 | - |
dc.contributor.localId | A00152 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A02300 | - |
dc.contributor.localId | A02873 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 28666022 | - |
dc.contributor.alternativeName | Koh, Hyoung Jun | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Yang, Woo Ick | - |
dc.contributor.alternativeName | Lee, Sung Chul | - |
dc.contributor.affiliatedAuthor | Koh, Hyoung Jun | - |
dc.contributor.affiliatedAuthor | Kim, Chan Yun | - |
dc.contributor.affiliatedAuthor | Yang, Woo Ick | - |
dc.contributor.affiliatedAuthor | Lee, Sung Chul | - |
dc.citation.volume | 12 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | e0180482 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.12(6) : e0180482, 2017 | - |
dc.identifier.rimsid | 39803 | - |
dc.type.rims | ART | - |
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