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Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells
DC Field | Value | Language |
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dc.contributor.author | 방난심 | - |
dc.contributor.author | 정일영 | - |
dc.date.accessioned | 2018-03-26T16:47:25Z | - |
dc.date.available | 2018-03-26T16:47:25Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2234-7658 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/156819 | - |
dc.description.abstract | OBJECTIVES: Sodium hypochlorite (NaOCl) is an excellent bactericidal agent, but it is detrimental to stem cell survival, whereas intracanal medicaments such as calcium hydroxide (Ca[OH]2) promote the survival and proliferation of stem cells. This study evaluated the effect of sequential NaOCl and Ca[OH]2 application on the attachment and differentiation of dental pulp stem cells (DPSCs). MATERIALS AND METHODS: DPSCs were obtained from human third molars. All dentin specimens were treated with 5.25% NaOCl for 30 min. DPSCs were seeded on the dentin specimens and processed with additional 1 mg/mL Ca[OH]2, 17% ethylenediaminetetraacetic acid (EDTA) treatment, file instrumentation, or a combination of these methods. After 7 day of culture, we examined DPSC morphology using scanning electron microscopy and determined the cell survival rate with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We measured cell adhesion gene expression levels after 4 day of culture and odontogenic differentiation gene expression levels after 4 wk using quantitative real-time polymerase chain reaction. RESULTS: DPSCs did not attach to the dentin in the NaOCl-treated group. The gene expression levels of fibronectin-1 and secreted phosphoprotein-1 gene in both the Ca[OH]2- and the EDTA-treated groups were significantly higher than those in the other groups. All Ca[OH]2-treated groups showed higher expression levels of dentin matrix protein-1 than that of the control. The dentin sialophosphoprotein level was significantly higher in the groups treated with both Ca[OH]2 and EDTA. CONCLUSIONS: The application of Ca[OH]2 and additional treatment such as EDTA or instrumentation promoted the attachment and differentiation of DPSCs after NaOCl treatment. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean | - |
dc.publisher | Korean Academy of Conservative Dentistry | - |
dc.relation.isPartOf | Restorative Dentistry & Endodontics | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effect of dentin treatment on proliferation and differentiation of human dental pulp stem cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Advanced General Dentistry | - |
dc.contributor.googleauthor | Minjeong Park | - |
dc.contributor.googleauthor | Nan-Sim Pang | - |
dc.contributor.googleauthor | Il-Young Jung | - |
dc.identifier.doi | 10.5395/rde.2015.40.4.290 | - |
dc.contributor.localId | A01783 | - |
dc.contributor.localId | A03700 | - |
dc.relation.journalcode | J02618 | - |
dc.identifier.eissn | 2234-7666 | - |
dc.identifier.pmid | 26587415 | - |
dc.subject.keyword | Calcium hydroxide | - |
dc.subject.keyword | Cell attachment | - |
dc.subject.keyword | Cell differentiation | - |
dc.subject.keyword | Dental pulp stem cells | - |
dc.subject.keyword | Regenerative endodontics | - |
dc.subject.keyword | Sodium hypochlorite | - |
dc.contributor.alternativeName | Pang, Nan Sim | - |
dc.contributor.alternativeName | Jung, Il Young | - |
dc.contributor.affiliatedAuthor | Pang, Nan Sim | - |
dc.contributor.affiliatedAuthor | Jung, Il Young | - |
dc.citation.volume | 40 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 290 | - |
dc.citation.endPage | 298 | - |
dc.identifier.bibliographicCitation | Restorative Dentistry & Endodontics, Vol.40(4) : 290-298, 2015 | - |
dc.identifier.rimsid | 39947 | - |
dc.type.rims | ART | - |
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