Cited 39 times in
Mitomycin-C Induces the Apoptosis of Human Tenon’s Capsule Fibroblast by Activation of c-Jun N-Terminal Kinase 1 and Caspase-3 Protease
DC Field | Value | Language |
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dc.contributor.author | 김찬윤 | - |
dc.contributor.author | 성공제 | - |
dc.contributor.author | 홍영재 | - |
dc.date.accessioned | 2017-10-26T06:41:28Z | - |
dc.date.available | 2017-10-26T06:41:28Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151181 | - |
dc.description.abstract | PURPOSE: To investigate whether mitochondrial dysfunction and mitogen-activated protein kinase family proteins are implicated in apoptotic signaling of human Tenon's capsule fibroblasts (HTCFs) by mitomycin-C. METHODS: Apoptosis was determined by Hoechst nuclei staining, agarose gel electrophoresis, and flow cytometry in HTCFs treated with 0.4 mg/mL mitomycin-C for 5 minutes. Enzymatic digestion of florigenic biosubstrate assessed the catalytic activity of caspase proteases, including caspase-3, caspase-8, and caspase-9. Phosphotransferase activity of c-Jun N-terminal kinase (JNK) 1 was measured by in vitro immune complex kinase assay using c-Jun(1-79) protein as a substrate. Mitochondrial membrane potential transition (MPT) was measured by flow cytometric analysis of JC-1 staining. RESULTS: Mitomycin-C (0.4 mg/mL) induced the apoptosis of HTCFs, which was characterized as nucleic acid and genomic DNA fragmentation, chromatin condensation, and sub-G(0)/G(1) fraction of cell cycle increase. The catalytic activity of caspase-3 and caspase-9 was significantly increased and was accompanied by cytosolic release of cytochrome c and MPT in response to mitomycin-C. Treatment with mitomycin-C resulted in the increased expression of Fas, FasL, Bad, and phosphorylated p53 and a decreased level of phosphorylated AKT. Treatment with mitomycin-C also increased the phosphotransferase activity and tyrosine phosphorylation of JNK1, whose inhibitor significantly suppressed the cytotoxicity of mitomycin-C. CONCLUSIONS: Mitomycin-C induced the apoptosis of HTCFs through the activation of intrinsic and extrinsic caspase cascades with mitochondrial dysfunction. It also activated Fas-mediated apoptotic signaling of fibroblasts. Furthermore, the activation of JNK1 played a major role in the cytotoxicity of mitomycin-C. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Mitomycin-C Induces the Apoptosis of Human Tenon’s Capsule Fibroblast by Activation of c-Jun N-Terminal Kinase 1 and Caspase-3 Protease | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Gong Je Seong | - |
dc.contributor.googleauthor | Channy Park | - |
dc.contributor.googleauthor | Chan Yoon Kim | - |
dc.contributor.googleauthor | Young Jae Hong | - |
dc.contributor.googleauthor | Hong-Seob So | - |
dc.contributor.googleauthor | Sang-Duck Kim | - |
dc.contributor.googleauthor | Raekil Park | - |
dc.identifier.doi | 10.1167/iovs.04-1358 | - |
dc.contributor.localId | A01035 | - |
dc.contributor.localId | A01946 | - |
dc.contributor.localId | A04416 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 10.1167/iovs.04-1358 | - |
dc.contributor.alternativeName | Kim, Chan Yun | - |
dc.contributor.alternativeName | Seong, Gong Je | - |
dc.contributor.alternativeName | Hong, Young Jae | - |
dc.citation.volume | 46 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 3545 | - |
dc.citation.endPage | 3552 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.46(10) : 3545-3552, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 43514 | - |
dc.type.rims | ART | - |
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