Cited 8 times in
Effects of Tautomycetin on Proliferation and Fibronectin Secretion in Vascular Smooth Muscle Cells and Glomerular Mesangial Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 강신욱 | - |
dc.contributor.author | 김유선 | - |
dc.date.accessioned | 2017-05-04T07:27:58Z | - |
dc.date.available | 2017-05-04T07:27:58Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147328 | - |
dc.description.abstract | Tautomycetin (TMC), a newly developed immunosuppressive agent, induces T-lymphocyte apoptosis through the inhibition of tyrosine kinase and protein phosphatase 1. We examined the effects of TMC on platelet-derived growth factor (PDGF)-induced proliferation and extracellular matrix synthesis in cultured vascular smooth muscle cells (VSMCs) and mesangial cells (MCs) of Sprague-Dawley rats, and investigated the molecular mechanisms involved. Different concentrations of TMC were administered 1 hour before the addition of 10 ng/mL PDGF into the growth-arrested and synchronized cells. Cell proliferation was assessed by methylthiazoletetrazolium (MTT) assay, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK by Western blot analysis. PDGF increased cell proliferation, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK in both VSMCs and MCs. In both cultured cells, TMC at >1 mug/mL significantly reduced basal MTT. TMC at 100 ng/mL significantly decreased the PDGF-induced VSMC and MC proliferation. However, fibronectin secretion and the activation of Akt, ERK, and p38 MAPK were not affected by this nontoxic concentration of TMC. The present data demonstrate that low-dose TMC reduced PDGF-induced VSMC and MC proliferation without affecting the fibronectin secretion and cellular kinase activation. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Science Inc. | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antifungal Agents/pharmacology* | - |
dc.subject.MESH | Cell Division/drug effects* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Extracellular Matrix/drug effects | - |
dc.subject.MESH | Extracellular Matrix/physiology | - |
dc.subject.MESH | Fibronectins/metabolism* | - |
dc.subject.MESH | Furans | - |
dc.subject.MESH | Glomerular Mesangium/drug effects | - |
dc.subject.MESH | Glomerular Mesangium/physiology* | - |
dc.subject.MESH | Lipids | - |
dc.subject.MESH | Muscle, Smooth, Vascular/drug effects | - |
dc.subject.MESH | Muscle, Smooth, Vascular/physiology* | - |
dc.subject.MESH | Platelet-Derived Growth Factor/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.title | Effects of Tautomycetin on Proliferation and Fibronectin Secretion in Vascular Smooth Muscle Cells and Glomerular Mesangial Cells | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.department | Dept. of Surgery (외과학교실) | - |
dc.contributor.googleauthor | J.H. Kim | - |
dc.contributor.googleauthor | T.Y. Lee | - |
dc.contributor.googleauthor | J. Park | - |
dc.contributor.googleauthor | H. Ha | - |
dc.contributor.googleauthor | S.-W. Kang | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2005.02.082 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00785 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 15919517 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041134505001995 | - |
dc.subject.keyword | 15919517 | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.citation.volume | 37 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1959 | - |
dc.citation.endPage | 1961 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.37(4) : 1959-1961, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 48500 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.