0 660

Cited 26 times in

Synergistic Action of IL-8 and Bone Marrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors.

Authors
 Dong Suk Yoon  ;  Kyoung-Mi Lee  ;  Sung-Hwan Kim  ;  Su Hee Kim  ;  Youngmee Jung  ;  Soo Hyun Kim  ;  Kwang Hwan Park  ;  Yoorim Choi  ;  Hyun Aae Ryu  ;  Woo Jin Choi  ;  Jin Woo Lee 
Citation
 TISSUE ENGINEERING PART A, Vol.22(3-4) : 363-374, 2016 
Journal Title
TISSUE ENGINEERING PART A
ISSN
 1937-3341 
Issue Date
2016
MeSH
Animals ; Bone Marrow/chemistry* ; Cartilage/physiology* ; Cell Differentiation/drug effects ; Chondrogenesis/drug effects* ; Dogs ; Interleukin-8/pharmacology* ; Knee Joint/physiology ; Male ; Mesenchymal Stromal Cells/metabolism* ; Regeneration/drug effects* ; Transcription Factors/metabolism*
Abstract
The objective of this study was to determine whether a biphasic scaffold loaded with a combination of a chemokine and bone marrow concentrate (BMC) could improve tissue regeneration in knee articular cartilage of beagles with cylindrical osteochondral defects. For this investigation, an osteochondral defect (6 mm in diameter and 8 mm deep) was created in the weight-bearing articular surface of the femoral medial condyle in beagles. Bone marrow was aspirated from the posterior iliac crests of beagles to obtain mesenchymal stem cells (MSCs) for in vitro assay. Hematoxylin and eosin (HE), Masson's trichrome (MT), safranin O/fast green staining, and immunohistochemistry were performed for histological analysis. Quantitative real-time polymerase chain reaction was performed to understand the roles of BMC in chondrogenic differentiation of MSCs. At 12 weeks after transplantation of biphasic scaffolds, we observed that interleukin-8 (IL-8) or the combination of IL-8 and BMC induced massive bone regeneration compared to saline, BMC only, and MSCs. In gross appearance, the osteochondral defect site was nearly completely filled with repair tissue in the group that received the combination of IL-8 and BMC but not in the other groups. Moreover, histological analysis showed obvious differences in cartilage regeneration among groups. HE and MT staining showed that the cartilage defect sites of the group receiving the combination of IL-8 and BMC were regenerated with cartilage-like tissues showing chondrocyte morphology. Safranin O staining showed hyaline cartilage regeneration in the group receiving IL-8 and BMC, whereas fibrous-like tissues were formed in the other groups. Furthermore, immunostaining revealed the presence of type II collagen and aggrecan in regenerated cartilage tissue of the group receiving IL-8 and BMC, whereas regenerated cartilage tissues of the other groups weakly expressed type II collagen and aggrecan. These results indicate that the combination of a chemokine IL-8 and BMC has significant positive effects on osteochondral regeneration in a beagle model through enhancing expression of the chondrogenic transcription factors and markers such as Sox9 and type II collagen.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/ten.tea.2015.0425
DOI
10.1089/ten.tea.2015.0425
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Hwan(김성환) ORCID logo https://orcid.org/0000-0001-5743-6241
Park, Kwang Hwan(박광환) ORCID logo https://orcid.org/0000-0002-2110-0559
Yoon, Dong Suk(윤동석) ORCID logo https://orcid.org/0000-0001-5945-5569
Lee, Kyoung Mi(이경미) ORCID logo https://orcid.org/0000-0002-9038-8162
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Choi, Woo Jin(최우진)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146765
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links