Cited 55 times in
Real-time monitoring of 3D cell culture using a 3D capacitance biosensor
DC Field | Value | Language |
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dc.contributor.author | 박용범 | - |
dc.contributor.author | 신전수 | - |
dc.contributor.author | 최인홍 | - |
dc.date.accessioned | 2017-02-24T03:32:10Z | - |
dc.date.available | 2017-02-24T03:32:10Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/146368 | - |
dc.description.abstract | Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.format.extent | 56~61 | - |
dc.publisher | Elsevier Advanced Technology | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Biological Assay/instrumentation* | - |
dc.subject.MESH | Biosensing Techniques/instrumentation* | - |
dc.subject.MESH | Cell Culture Techniques/instrumentation* | - |
dc.subject.MESH | Cell Movement/physiology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Computer Systems | - |
dc.subject.MESH | Conductometry/instrumentation* | - |
dc.subject.MESH | Electric Capacitance | - |
dc.subject.MESH | Equipment Design | - |
dc.subject.MESH | Equipment Failure Analysis | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | MCF-7 Cells | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology | - |
dc.subject.MESH | Mesenchymal Stromal Cells/physiology* | - |
dc.subject.MESH | Monitoring, Physiologic/instrumentation* | - |
dc.title | Real-time monitoring of 3D cell culture using a 3D capacitance biosensor | - |
dc.type | Article | - |
dc.publisher.location | England | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Internal Medicine | - |
dc.contributor.googleauthor | Sun-Mi Lee | - |
dc.contributor.googleauthor | Nalae Han | - |
dc.contributor.googleauthor | Rimi Lee | - |
dc.contributor.googleauthor | In-Hong Choi | - |
dc.contributor.googleauthor | Yong-Beom Park | - |
dc.contributor.googleauthor | Jeon-Soo Shin | - |
dc.contributor.googleauthor | Kyung-Hwa Yoo | - |
dc.identifier.doi | 10.1016/j.bios.2015.09.005 | - |
dc.contributor.localId | A04167 | - |
dc.contributor.localId | A01579 | - |
dc.contributor.localId | A02144 | - |
dc.relation.journalcode | J00330 | - |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.pmid | 26386332 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0956566315304024 | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Capacitance sensor | - |
dc.subject.keyword | Migration | - |
dc.subject.keyword | Proliferation | - |
dc.subject.keyword | Real-time monitoring | - |
dc.subject.keyword | Three-dimensional (3D) cell culture | - |
dc.contributor.alternativeName | Park, Yong Beom | - |
dc.contributor.alternativeName | Shin, Jeon Soo | - |
dc.contributor.alternativeName | Choi, In Hong | - |
dc.contributor.affiliatedAuthor | Choi, In Hong | - |
dc.contributor.affiliatedAuthor | Park, Yong Beom | - |
dc.contributor.affiliatedAuthor | Shin, Jeon Soo | - |
dc.citation.volume | 77 | - |
dc.citation.startPage | 56 | - |
dc.citation.endPage | 61 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, Vol.77 : 56-61, 2016 | - |
dc.date.modified | 2017-02-24 | - |
dc.identifier.rimsid | 47877 | - |
dc.type.rims | ART | - |
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