404 438

Cited 0 times in

Comparison of oral microbiome in siblings using next generation sequencing

Authors
 이효설 
Issue Date
2015
Description
치의학과/석사
Abstract
The purpose of this study was to identify the oral microbiome in siblings with or without dental caries using next generation sequencing to verify the ecological changes in health and caries state and to identify the horizontal transmission. To investigate the composition of microbiome, 14 young siblings including 7 in caries group and 7 in caries-free group (average of 4.3 years) were enrolled in 7 families. Supragingival plaques were collected from cervico-buccal area of posterior teeth. All samples were analyzed by pyrosequencing based on 16S rRNA gene V1-V4 hypervariable regions. At the phylum level of caries-group, Firmicutes increased and Proteobacteria, Bacteroidetes, Fusobacteria, and Actinobacteria decreased. At the genus level of caries-group, Streptococcus increased and Haemophilus, Capnocytophage, and Leptotrichia decreased. At species level of caries group, Veillonella dispar, Veillonella tobetsuensis, Veillonella rodentium, Actinomyces viscosus, and Streptococcus sanguinis increased and Streptococcus mitis, Capnocytophage sputiger, Capnocytophage leadbeltt and AF366267 decreased. Similarity between the siblings was evident in UniFrac distance (p<0.05). In conclusion, these results showed the ecological change of oral microbiome in caries, the same as the previous researches. In addition, it appeared that the horizontal transmission contributed to the colonization of oral microbiome in siblings. We suggested that the strategy of caries prevention and management have to be considered in more holistic and personalized approach.
Files in This Item:
T013500.pdf Download
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 2. Thesis
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146172
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links