Macrophage와 Glycated-LDL의 Uptake 및 산화에 미치는 몇 가지 Effector의 효과

Abstract

Objective: Diabetes is associated with accelerated atherosclerosis, with the incidence of cardiovascular disease in diabetic patients, three to four times that of nondiabetic individuals. Glucose reacts with LDL in a process known as nonenzymatic glycation. Also, oxidation of LDL is a factor in the develomemt of atheroclerosis. From the previous study, we found that M-CSF and PMA increased the viability of macrophage while LPS and dexamethasone decreased. In the present study, effects of effectors on uptake and oxidation of glycated-LDL on HMDM(human monocyte derived macrophage) were tested.

Methods: Glycated-LDL was labelled with 5-iodoacetamide(5-IAF) in order to investigate both binding and degradation of glycated-LDL by human monocyte derived macrophage. Also, the electrophoretic mobility and thiobarbituric acid reactive substance(TBARS) value of glycated-LDL were compared.

Results: From the experimental results on the effects of target molecules on glyco-oxidation of LDL, M-CSF and PMA enhanced the LDL glycoxidation and mobilities on agarose gel electrophoresis whereas LPS and dexamethasone decreased. Also, effects of effectors on the viability of macrophage and uptake of glycated-LDL showed the same results from the glycoxidation of LDL.

Conclusion: Finally, we concluded that effects of effectors on the viability of macrophage and uptake of glycated-LDL showed the same pattern compared to the glycoxidation of LDL. And it appears that factors such as viability, macrophage uptake, and glycoxidation are related on the development of atherosclerosis.