Cited 8 times in
Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum
DC Field | Value | Language |
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dc.contributor.author | 박효진 | - |
dc.date.accessioned | 2016-05-16T11:09:48Z | - |
dc.date.available | 2016-05-16T11:09:48Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0944-1174 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143930 | - |
dc.description.abstract | BACKGROUND:Endotoxin modulates esophageal motor function by increasing nitric oxide (NO) production. The aims of this study were to examine inducible nitric oxide synthase (iNOS) induction in the lower esophageal sphincter (LES) of endotoxemic opossums and to investigate the effects of aminoguanidine (AG), a selective inhibitor of iNOS, on plasma nitrite/nitrate levels and on iNOS protein and mRNA expression after exposure to lipopolysaccharide (LPS). METHODS: Before and 12 h after the intravenous administration of LPS and/or AG, plasma nitrite/nitrate levels were determined. The iNOS protein and mRNA expression were investigated in the tissues taken from the LES by Western blot and reverse-transcriptase polymerase chain reaction (RT-PCR). RESULTS: Plasma nitrite/nitrate levels were significantly increased by LPS. The increase in plasma nitrite/nitrate produced by LPS was significantly decreased by AG. Western blot and RT-PCR demonstrated that iNOS expression was markedly increased by LPS, and attenuated slightly by AG. CONCLUSION: These studies support the hypothesis that endotoxin increases NO production by the induction of iNOS protein and mRNA. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1000~1004 | - |
dc.relation.isPartOf | JOURNAL OF GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Endotoxemia/enzymology* | - |
dc.subject.MESH | Endotoxemia/physiopathology* | - |
dc.subject.MESH | Esophagogastric Junction/drug effects* | - |
dc.subject.MESH | Esophagogastric Junction/physiopathology* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Guanidines/pharmacology* | - |
dc.subject.MESH | Lipopolysaccharides | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nitric Oxide/analysis | - |
dc.subject.MESH | Nitric Oxide/metabolism | - |
dc.subject.MESH | Nitric Oxide Synthase/genetics | - |
dc.subject.MESH | Nitric Oxide Synthase/metabolism* | - |
dc.subject.MESH | Nitric Oxide Synthase Type II | - |
dc.subject.MESH | Opossums | - |
dc.subject.MESH | Probability | - |
dc.subject.MESH | RNA, Messenger/analysis | - |
dc.subject.MESH | Random Allocation | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Statistics, Nonparametric | - |
dc.title | Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Hyojin Park | - |
dc.contributor.googleauthor | Eugene Clark | - |
dc.contributor.googleauthor | Joseph J. Cullen | - |
dc.contributor.googleauthor | John G. Koland | - |
dc.contributor.googleauthor | Myong Soo Kim | - |
dc.contributor.googleauthor | Jeffrey L. Conklin | - |
dc.identifier.doi | 10.1007/s005350200169 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01774 | - |
dc.relation.journalcode | J01416 | - |
dc.identifier.eissn | 1435-5922 | - |
dc.identifier.pmid | 12522530 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs005350200169 | - |
dc.contributor.alternativeName | Park, Hyo Jin | - |
dc.contributor.affiliatedAuthor | Park, Hyo Jin | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 37 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1000 | - |
dc.citation.endPage | 1004 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTROENTEROLOGY, Vol.37(12) : 1000-1004, 2002 | - |
dc.identifier.rimsid | 39517 | - |
dc.type.rims | ART | - |
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