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IL-13 suppresses MUC5AC gene expression and mucin secretion in nasal epithelial cell

Authors
 Chang Hoon Kim  ;  Kyoung Seob Song  ;  Ja Seok Koo  ;  Hyun Ung Kim  ;  Jae Young Choi  ;  Hyun Jun Kim  ;  Joo Heon Yoon 
Citation
 ACTA OTO-LARYNGOLOGICA, Vol.122(6) : 638-643, 2002 
Journal Title
ACTA OTO-LARYNGOLOGICA
ISSN
 0001-6489 
Issue Date
2002
MeSH
Cell Count ; Cells, Cultured ; Dose-Response Relationship, Drug ; Humans ; Immunohistochemistry ; Interleukin-13/pharmacology* ; Interleukin-13/physiology ; Mucin 5AC ; Mucin-2 ; Mucins/biosynthesis ; Mucins/genetics* ; Mucins/metabolism* ; Nasal Mucosa/cytology ; Nasal Mucosa/metabolism ; Nasal Mucosa/physiopathology* ; RNA, Messenger/analysis ; Reverse Transcriptase Polymerase Chain Reaction
Keywords
Mucin ; Gene ; Expression ; Mucin ; Secretion
Abstract
Recently, it was found that IL-13 is involved in allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. After treatment with IL-13, total mucin and MUC5AC mucin levels were measured using an immunoblotting assay. MUC2, MUC5AC and MUC8 mRNA expression were determined using reverse transcription-polymerase chain reaction. Also, immunostaining was performed using a MUC5AC antibody on histologic and cytospin slides. After treatment with > or = 5 ng/ml IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed by immunostaining. These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
Full Text
http://www.tandfonline.com/doi/abs/10.1080/000164802320396321
DOI
10.1080/000164802320396321
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Song, Kyoung Seob(송경섭)
Yoon, Joo Heon(윤주헌)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143873
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