Evaluation of Efficiency of Screening Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Hospitals Where the Bacteria Are Increasingly Prevalent

K. Lee; J. K. Lim; D. Yong; J. Yum; Y. Chong; R. Okamoto; M. Inoue

doi:10.1128/JCM.39.10.3696-3699.2001

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Evaluation of Efficiency of Screening Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Hospitals Where the Bacteria Are Increasingly Prevalent

Authors: K. Lee ; J. K. Lim ; D. Yong ; J. Yum ; Y. Chong ; R. Okamoto ; M. Inoue

Citation: JOURNAL OF CLINICAL MICROBIOLOGY, Vol.39(10) : 3696-3699, 2001

Journal Title: JOURNAL OF CLINICAL MICROBIOLOGY

ISSN: 0095-1137

Issue Date: 2001

MeSH: Anti-Bacterial Agents/pharmacology* ; Cefotaxime/pharmacology ; Ceftazidime/pharmacology ; Clavulanic Acid/pharmacology ; Drug Stability ; Escherichia coli/drug effects* ; Escherichia coli/enzymology ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Hospitals ; Humans ; Klebsiella Infections/epidemiology ; Klebsiella Infections/microbiology ; Klebsiella pneumoniae/drug effects* ; Klebsiella pneumoniae/enzymology ; Microbial Sensitivity Tests/methods ; Microbial Sensitivity Tests/standards ; Prevalence ; beta-Lactamases/biosynthesis* ; beta-Lactamases/drug effects

Abstract: The disk screening methods for extended-spectrum β-lactamase-producing strains were evaluated. The confirmatory work is reduced significantly in settings such as those in this study, by changing the cefpodoxime breakpoint to ≤20 mm and by not testing cefoxitin-resistant isolates. Cefotaxime and ceftazidime disk screening is reliable, and the laboratory-prepared cefotaxime- and ceftazidime-clavulanic acid disks are stable at −20°C for 12 weeks.