Cited 17 times in
Effect of bradykinin on cultured bovine corneal endothelial cells
DC Field | Value | Language |
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dc.contributor.author | 이운규 | - |
dc.date.accessioned | 2016-02-19T11:06:54Z | - |
dc.date.available | 2016-02-19T11:06:54Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0030-3755 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142429 | - |
dc.description.abstract | PURPOSE: To clarify the effect of bradykinin on cytosolic free calcium mobilization and cell proliferation in cultured bovine corneal endothelial cells (BCEC). METHODS: The cytosolic free calcium concentration (Ca2+]i) was measured with the InCa(TM) Imaging System after the treatment of bradykinin (10(-11) to 10(-7) M) alone or with the pretreatments of EGTA, bradykinin receptor (Bk1 and Bk2) antagonists and an inhibition of phospholipase C (U-73122). Also, the effect of bradykinin on cell proliferation in BCEC was evaluated using cell counts. RESULTS: In BCEC, [Ca2+]i in the resting state was 87 +/- 9 nM. Bradykinin induced an increment of [Ca2+]i in a concentration-dependent manner and its 50% effective concentration was approximately 5 x 10(-11) M. A [Ca2+]i increment at 10(-8) M bradykinin was inhibited with the pretreatment of EGTA, an extracellular calcium chelator. U-73122 (5 x 10(-6) M) attenuated the bradykinin-induced [Ca2+]i increment. The pretreatment of HOE-140 (Bk2 antagonist) almost attenuated the bradykinin (10(-8) M)-induced [Ca2+]i increase, but des-Arg9-[Leu(8)]-bradykinin (Bk1 antagonist) did not suppress it. To investigate the physiological effect of bradykinin, the effect of bradykinin on cell proliferation was studied. 10(-8) M of bradykinin produced a significant increase in cell numbers. This mitogenic effect of bradykinin was inhibited by the Bk2 antagonist. CONCLUSIONS: Bradykinin-induced stimulation of the signal transduction pathway in BCEC is coupled with the Bk2 type receptor. Furthermore, bradykinin produces the mitogenic effect in BCEC. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 303~308 | - |
dc.relation.isPartOf | OPHTHALMOLOGICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Bradykinin/analogs & derivatives | - |
dc.subject.MESH | Bradykinin/pharmacology* | - |
dc.subject.MESH | Bradykinin Receptor Antagonists | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Cattle | - |
dc.subject.MESH | Cell Count | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Egtazic Acid/pharmacology | - |
dc.subject.MESH | Endothelium, Corneal/cytology | - |
dc.subject.MESH | Endothelium, Corneal/drug effects* | - |
dc.subject.MESH | Endothelium, Corneal/metabolism | - |
dc.subject.MESH | Estrenes/pharmacology | - |
dc.subject.MESH | Inositol 1,4,5-Trisphosphate/metabolism | - |
dc.subject.MESH | Pyrrolidinones/pharmacology | - |
dc.subject.MESH | Receptor, Bradykinin B2 | - |
dc.subject.MESH | Receptors, Bradykinin/metabolism | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Type C Phospholipases/antagonists & inhibitors | - |
dc.subject.MESH | Type C Phospholipases/metabolism | - |
dc.title | Effect of bradykinin on cultured bovine corneal endothelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Yang S.W. | - |
dc.contributor.googleauthor | Lee W.K. | - |
dc.contributor.googleauthor | Lee E.J. | - |
dc.contributor.googleauthor | Kim K.Y. | - |
dc.contributor.googleauthor | Lim Y. | - |
dc.contributor.googleauthor | Lee K.H. | - |
dc.contributor.googleauthor | Rha H.K. | - |
dc.contributor.googleauthor | Hahn T.W. | - |
dc.identifier.doi | 10.1159/000050879 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02996 | - |
dc.relation.journalcode | J02431 | - |
dc.identifier.eissn | 1423-0267 | - |
dc.identifier.pmid | 11399940 | - |
dc.identifier.url | http://www.karger.com/Article/FullText/50879 | - |
dc.subject.keyword | Bradykinin | - |
dc.subject.keyword | Bovine corneal endothelial cell | - |
dc.subject.keyword | Calcium mobilization | - |
dc.subject.keyword | Bk2 receptor | - |
dc.contributor.alternativeName | Lee, Woon Kyu | - |
dc.contributor.affiliatedAuthor | Lee, Woon Kyu | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 215 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 303 | - |
dc.citation.endPage | 308 | - |
dc.identifier.bibliographicCitation | OPHTHALMOLOGICA, Vol.215(4) : 303-308, 2001 | - |
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