기니픽에서 라미닌이 함유된 감염방지형 교원질막의 창상치유 효과

Abstract

Biocompatibility of a material applied to wounded tissue is a prerequisite for optimal wound environment. Collagen, the major protein of the ECM, has been used clinically as an effective biomaterial for wound healing and skin regeneration due to high biocompatibility, low antigenicity, low toxicity and hemostatic capability. We intended to design a laminin (Lam) coated/infection- preventive bilayered collagen membrane containing silver sulfadiazind (AgSD) loaded hyaluronic acid microparticles, able to prevent rapid biodegradation/infection and to improve wound healing.

When comparing the effect of a laminin coated/infection- preventive bilayered collagen membrane on reduction of wound size in guinea pig with polyurethane film, silver sulfadiazine medicated collagen membranes (Lam(+)/AgSD(+), Lam(-)/AgSD(+)) demonstrated significantly higher reduction of wound size on 3, 7 and 14 days after application. When evaluating the effect of addition of laminin to antibiotics medicated collagen membrane on reduction of wound size, Lam(+)/AgSD(+) membrane demonstrated significantly higher reduction of wound size than Lam(-)/AgSD(+) membrane on 3 days after application. When evaluating the effect of addition of silver sulfadiazine to laminin coated collagen membrane on wound size than Lam(+)/AgSD(+), membrane demonstrated significantly higher reduction of wound size than Lam(+)/AgSD(-) membrane on 3 days after application. On histologic examination, laminin coated/AgSD medicated collagen membrane demonstrated faster new vascularization, more fibroblast proliferation and less inflammation than control.

These results suggested that laminin coated/infection medicated collagen membrane synergistically improved wound healing.