고온 열처리한 돼지 해면골의 화학, 구조적 평가와 골형성능에 대한 연구

Abstract

Purpose: The purpose of this study is to determine the efficacy of bioactive hydroxyapatite obtained from the porcine cancellous bone for the treatment of bone defect and nonunion. Materials and Methods: Porcine cancellous bones were heat-treated at 1300℃ for 2 hours. The chemical compositions, calcium to phosphate ratio and microstructures of mineralized porcine bone were examined. For in vivo implantation, bone defects were made on the anteromedial aspects of proximal tibia in 7 beagle dogs and theses artificial bones were inserted. Plain X-ray was taken at every 2 weeks interval for radiologic evaluation. At 12 weeks, specimens were evaluated histologyically with hematoxylin and eosin stain. Results: The composition and morphology of mineralized porcine cancellous bone were similar to those of heat-treated human cancellous bone. Radiographs showed union at the host bone-bone block interfaces. At 12 weeks, all uniform and substantial new bone formation were observed. Conclusion: This mineralization technique has several advantages such as no disease transmission, no immune reaction, excellent biocompatibility, and cost-effectiveness. Consequently, mineralized porcine cancellous bone showed an effective osteoconductivity.