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HNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene

Title
HNF1 and/or HNF3 may contribute to the tissue specific expression of glucokinase gene
Authors
Ji-Young Cha;Ha-il Kim;Yong-ho Ahn;Tian-Zhu Li;Seung-Soon Im
Issue Date
2001
Journal Title
Experimental and Molecular Medicine
ISSN
1226-3613
Citation
Experimental and Molecular Medicine, Vol.33(2) : 59~63, 2001
Abstract
A possible role of hepatocyte nuclear factor 1 (HNF1) or HNF3, a predominant trans-acting factors of hepatic or pancreatic β-cells, was examined on the tissue specific interdependent expression of glucokinase (GK) in liver, H4IIE, HepG2, HIT-T15 and MIN6 cell line. The tissues or cell lines known to express GK showed abundant levels of HNF1 and HNF3 mRNA as observed in liver, H4IIE, HepG2, HIT-T15 and MIN6 cells, whereas they were not detected in brain, heart, NIH 3T3, HeLa cells. The promoter of glucokinase contains several HNF3 consensus sequences and are well conserved in human, mouse and rat. Transfection of the glucokinase promotor linked with luciferase reporter to liver or pancreatic β cell lines showed high interacting activities with HNF1 and HNF3, whereas minimal activities were detected in the cells expressing very low levels of HNFs. The binding of HNF1 or HNF3 to the GK promoter genes was confirmed by electrophoretic mobility shift assay (EMSA). From these data, we propose that the expression of HNF1 and/or HNF3 may, in part, contribute to the tissue specific expression of GK.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/142106
DOI
10.1038/emm.2001.11
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Biochemistry & Molecular Biology
Yonsei Authors
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