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Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes

Title
 Biocompatibility Evaluation of Dental Luting Cements Using Cytokine Released from Human Oral Fibroblasts and Keratinocytes
Authors
 Jae-Sung Kwon; Yin-Zhu Piao; Kwang-Mahn Kim; Susun An; Ji Hoon Kim; Song-Yi Yang; Sun-A Cho
Issue Date
2015
Journal Title
 Materials
ISSN
 1996-1944
Citation
 Materials, Vol.8(11) : 7269~7277, 2015
Abstract
Dental luting cements are commonly used in dentistry for cementation of prosthetic restoration. Many previous studies focused on the measurement of the cell viability as the method of cytotoxicity evaluation during biocompatibility study for the material. In this study, the biocompatibility of various dental luting cements were evaluated using the new method of cytokine release measurement in order to better simulate inflammatory reactions in animal or clinical model using two different oral cells; immortalized human gingival fibroblast and immortalized human oral keratinocytes. Cells were exposed to extractions of various commercially available dental luting cements for different durations. Cytokines of IL-1α and IL-8 were measured from the supernatants of the cells and the results were then compared to the conventional MTT viability test. The result from the conventional cell viability study showed a relatively simple and straight forward indication that only one of the dental luting cements tested in this study was cytotoxic with increasing duration of exposure for both cells. Meanwhile, the result from the cytokine measurement study was much more complex at the time point they were measured, type of cells used for the study and the type of cytokines measured, all of which influenced the interpretation of the results. Hence, the better understanding of the cytokine release would be required for the application in biocompatibility evaluation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/141770
DOI
10.3390/ma8115372
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Dental Engineering
Yonsei Authors
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