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Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

Title
Real-time monitoring of 3D cell culture using a 3D capacitance biosensor
Authors
Sun-Mi Lee;Nalae Han;Kyung-Hwa Yoo;Jeon-Soo Shin;Yong-Beom Park;In-Hong Choi;Rimi Lee
Issue Date
2015
Journal Title
Biosensors & Bioelectronics
ISSN
0956-5663
Citation
Biosensors & Bioelectronics, Vol.77(null) : 56~61, 2015
Abstract
Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
URI
http://www.sciencedirect.com/science/article/pii/S0956566315304024

http://ir.ymlib.yonsei.ac.kr/handle/22282913/141765
DOI
10.1016/j.bios.2015.09.005
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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