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Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

Title
 Real-time monitoring of 3D cell culture using a 3D capacitance biosensor 
Authors
 Sun-Mi Lee ; Nalae Han ; Kyung-Hwa Yoo ; Jeon-Soo Shin ; Yong-Beom Park ; In-Hong Choi ; Rimi Lee 
Issue Date
2015
Journal Title
 Biosensors & Bioelectronics 
ISSN
 0956-5663 
Citation
 Biosensors & Bioelectronics, Vol.77(null) : 56~61, 2015 
Abstract
Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/141765
DOI
10.1016/j.bios.2015.09.005
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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Link
 http://www.sciencedirect.com/science/article/pii/S0956566315304024
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