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Expression and Localization of Aquaporin Water Channels in Human Middle Ear Epithelium.

Title
Expression and Localization of Aquaporin Water Channels in Human Middle Ear Epithelium.
Authors
Seo, Young Joon;Choi, Jae Young
Issue Date
2015
Journal Title
Otology & Neurotology
ISSN
1531-7129
Citation
Otology & Neurotology, Vol.36(7) : 1284~1289, 2015
Abstract
BACKGROUND: Although aquaporins (AQPs) are known to play critical roles as the basis for water and solute transport in water homeostasis, AQPs in normal human middle ear epithelium (NHMEE) has not previously been investigated. OBJECTIVES/HYPOTHESIS: To investigate the expressions of AQP water channels in NHMEE in situ, in proliferating epithelial cell cultures in vitro. METHODS: AQP 0-12 expressions by cultured NHMEE cells in situ were assessed by reverse transcriptase-polymerase chain reaction. Normal middle ear epithelial tissue was harvested and investigated for expressions of AQPs (1, 3, 4, and 5) by immunohistochemistry. Expression screening was also carried out on the differentiated NHMEE cells. RESULTS: Transcripts for AQP 1, 2, 3, 4, 5, 6, 8, 10, and 11 were expressed consistently in cultured NHMEE cells; however, AQP 0, 7, 9, and 12 subtypes were not expressed. Immunochemistry confirmed the expressions of AQP 1, 3, and 5 at the protein level. AQP 1 was localized at capillary endothelial cells and fibroblasts in lamina propria mucosae; AQP 3 was present solely at the basolateral membrane of ciliated cells, whereas AQP 5 was on the apical surface of ciliated cells. AQP 3 and 5 were intensely expressed in both cultured NHMEE cells in situ and NHMEE tissue in vitro. CONCLUSION: This is the first study to demonstrate that AQPs are expressed by human middle ear epithelium in situ and in vitro, suggesting a potential role in otitis media with effusion. Our study suggests that the presence of AQP 1, 3, and 5 in the middle ear cavity may be to have an important role for water transportation.
URI
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00129492-201508000-00023&LSLINK=80&D=ovft

http://ir.ymlib.yonsei.ac.kr/handle/22282913/141763
DOI
10.1097/MAO.0000000000000797.
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
Yonsei Authors
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