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Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide

Title
Stimulated Osteogenic Differentiation of Human Mesenchymal Stem Cells by Reduced Graphene Oxide
Authors
Jin, Linhua;Lee, Jong Ho;Han, Dong-Wook;Park, Jong-Chul;Lee, Mi Hee;Hong, Suck Won;Kim, Min Jeong;Shin, Yong Cheol;Jin, Oh Seong
Issue Date
2015
Journal Title
Journal of Nanoscience and Nanotechnology
ISSN
1533-4880
Citation
Journal of Nanoscience and Nanotechnology, Vol.15(10) : 7966~7970, 2015
Abstract
Osteoprogenitor cells play a significant role in the growth or repair of bones, and have great potential as cell sources for regenerative medicine and bone tissue engineering, but control of their specific differentiation into bone cells remains a challenge. Graphene-based nanomaterials are attractive candidates for biomedical applications as substrates for stem cell (SC) differentiation, scaffolds in tissue engineering, and components of implant devices owing to their biocompatible, transferable and implantable properties. This study examined the enhanced osteogenic differentiation of human mesenchymal stem cells (hMSCs) by reduced graphene oxide (rGO) nanoparticles (NPs), and rGO NPs was prepared by reducing graphene oxide (GO) with a hydrazine treatment followed by annealing in argon and hydrogen. The cytotoxicity profile of each particle was examined using a water-soluble tetrazolium-8 (WST-8) assay. At different time-points, a WST-8 assay, alkaline phosphatase (ALP) activity assay and alizarin red S (ARS) staining were used to determine the effects of rGO NPs on proliferation, differentiation and mineralization, respectively. The results suggest that graphene-based materials have potential as a platform for stem cells culture and biomedicalapplications.
URI
http://www.ingentaconnect.com/content/asp/jnn/2015/00000015/00000010/art00104

http://ir.ymlib.yonsei.ac.kr/handle/22282913/141692
DOI
10.1166/jnn.2015.11223
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Medical Engineering
Yonsei Authors
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