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A novel siderophore system is essential for the growth of Pseudomonas aeruginosa in airway mucus

Authors
 Mia Gi ; Kang-Mu Lee ; Jae Young Choi ; Sang Sun Yoon ; Joo-Heon Yoon ; Sang Cheol Kim 
Citation
 Scientific Reports, Vol.5(null) : 14644, 2015 
Journal Title
 Scientific Reports 
ISSN
 2045-2322 
Issue Date
2015
Abstract
Pseudomonas aeruginosa establishes airway infections in Cystic Fibrosis patients. Here, we investigate the molecular interactions between P.aeruginosa and airway mucus secretions (AMS) derived from the primary cultures of normal human tracheal epithelial (NHTE) cells. PAO1, a prototype strain of P. aeruginosa, was capable of proliferating during incubation with AMS, while all other tested bacterial species perished. A PAO1 mutant lacking PA4834 gene became susceptible to AMS treatment. The ΔPA4834 mutant was grown in AMS supplemented with 100 μM ferric iron, suggesting that the PA4834 gene product is involved in iron metabolism. Consistently, intracellular iron content was decreased in the mutant, but not in PAO1 after the AMS treatment. Importantly, a PAO1 mutant unable to produce both pyoverdine and pyochelin remained viable, suggesting that these two major siderophore molecules are dispensable for maintaining viability during incubation with AMS. The ΔPA4834 mutant was regrown in AMS amended with 100 μM nicotianamine, a phytosiderophore whose production is predicted to be mediated by the PA4836 gene. Infectivity of the ΔPA4834 mutant was also significantly compromised in vivo. Together, our results identify a genetic element encoding a novel iron acquisitionsystem that plays a previously undiscovered role in P. aeruginosa airway infection.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/141338
DOI
10.1038/srep14644.
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
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