Cited 16 times in
Rh D blood group conversion using transcription activator-like effector nucleases
DC Field | Value | Language |
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dc.contributor.author | 김현옥 | - |
dc.contributor.author | 김형범 | - |
dc.date.accessioned | 2016-02-04T11:28:23Z | - |
dc.date.available | 2016-02-04T11:28:23Z | - |
dc.date.issued | 2015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/140500 | - |
dc.description.abstract | Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a 12-transmembrane domain protein. Here we convert Rh D-positive erythroid progenitor cells into D-negative cells using RHD-targeting transcription activator-like effector nucleases (TALENs). After transfection of TALEN-encoding plasmids, RHD-knockout clones are obtained. Erythroid-lineage cells differentiated from these knockout erythroid progenitor cells do not agglutinate in the presence of anti-D reagents and do not express D antigen, as assessed using flow cytometry. Our programmable nuclease-induced blood group conversion opens new avenues for compatible donor cell generation in transfusion medicine. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Deoxyribonucleases/genetics* | - |
dc.subject.MESH | Erythrocytes/metabolism* | - |
dc.subject.MESH | Erythroid Precursor Cells/metabolism* | - |
dc.subject.MESH | Fetal Blood | - |
dc.subject.MESH | Gene Knockout Techniques/methods* | - |
dc.subject.MESH | Gene Transfer Techniques* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Induced Pluripotent Stem Cells | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | RNA, Messenger/metabolism* | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Rh-Hr Blood-Group System/genetics* | - |
dc.subject.MESH | Rh-Hr Blood-Group System/metabolism | - |
dc.subject.MESH | Transfection | - |
dc.title | Rh D blood group conversion using transcription activator-like effector nucleases | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Young-Hoon Kim | - |
dc.contributor.googleauthor | Hyun O. Kim | - |
dc.contributor.googleauthor | Eun J. Baek | - |
dc.contributor.googleauthor | Ryo Kurita | - |
dc.contributor.googleauthor | Hyuk-Jin Cha | - |
dc.contributor.googleauthor | Yukio Nakamura | - |
dc.contributor.googleauthor | Hyongbum Kim | - |
dc.identifier.doi | 10.1038/ncomms8451 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01122 | - |
dc.contributor.localId | A01148 | - |
dc.relation.journalcode | J02293 | - |
dc.identifier.eissn | 2041-1723 | - |
dc.identifier.pmid | 26078220 | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | Kim, Hyongbum | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 6 | - |
dc.citation.startPage | 7451 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, Vol.6 : 7451, 2015 | - |
dc.identifier.rimsid | 29943 | - |
dc.type.rims | ART | - |
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