Cited 31 times in
Evaluation of VITEK mass spectrometry (MS), a matrix-assisted laser desorption ionization time-of-flight MS system for identification of anaerobic bacteria
DC Field | Value | Language |
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dc.contributor.author | 용동은 | - |
dc.contributor.author | 이경원 | - |
dc.contributor.author | 정석훈 | - |
dc.contributor.author | 정윤섭 | - |
dc.date.accessioned | 2016-02-04T10:53:39Z | - |
dc.date.available | 2016-02-04T10:53:39Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/139212 | - |
dc.description.abstract | BACKGROUND: By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMérieux, France) in the identification of anaerobes. METHODS: We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing. RESULTS: Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level. CONCLUSIONS: The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 69~75 | - |
dc.relation.isPartOf | ANNALS OF LABORATORY MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bacteria, Anaerobic/genetics* | - |
dc.subject.MESH | Bacteria, Anaerobic/isolation & purification | - |
dc.subject.MESH | Bacterial Typing Techniques/instrumentation* | - |
dc.subject.MESH | Bacterial Typing Techniques/methods* | - |
dc.subject.MESH | Body Fluids/microbiology | - |
dc.subject.MESH | Databases, Genetic | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | RNA, Ribosomal, 16S/analysis* | - |
dc.subject.MESH | RNA, Ribosomal, 16S/metabolism | - |
dc.subject.MESH | Sequence Analysis, DNA | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* | - |
dc.title | Evaluation of VITEK mass spectrometry (MS), a matrix-assisted laser desorption ionization time-of-flight MS system for identification of anaerobic bacteria | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Wonmok Lee | - |
dc.contributor.googleauthor | Myungsook Kim | - |
dc.contributor.googleauthor | Dongeun Yong | - |
dc.contributor.googleauthor | Seok Hoon Jeong | - |
dc.contributor.googleauthor | Kyungwon Lee | - |
dc.contributor.googleauthor | Yunsop Chong | - |
dc.identifier.doi | 10.3343/alm.2015.35.1.69 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02423 | - |
dc.contributor.localId | A02649 | - |
dc.contributor.localId | A03619 | - |
dc.contributor.localId | A03679 | - |
dc.relation.journalcode | J00164 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.identifier.pmid | 25553283 | - |
dc.subject.keyword | Anaerobic bacteria | - |
dc.subject.keyword | MALDI-TOF MS | - |
dc.subject.keyword | VITEK MS | - |
dc.contributor.alternativeName | Yong, Dong Eun | - |
dc.contributor.alternativeName | Lee, Kyung Won | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.alternativeName | Chong, Yun Sop | - |
dc.contributor.affiliatedAuthor | Yong, Dong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Kyung Won | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Chong, Yun Sop | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 35 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 69 | - |
dc.citation.endPage | 75 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, Vol.35(1) : 69-75, 2015 | - |
dc.identifier.rimsid | 45495 | - |
dc.type.rims | ART | - |
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