Cited 17 times in
Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis.
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2015-12-28T11:03:22Z | - |
dc.date.available | 2015-12-28T11:03:22Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0732-8893 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/138596 | - |
dc.description.abstract | Rapid, accurate detection of Mycobacterium tuberculosis is crucial in the diagnosis of tuberculosis (TB), but conventional diagnostic methods have limited sensitivity and specificity or are time consuming. A new highly sensitive nucleic acid amplification test, combined nested and real-time polymerase chain reaction (PCR) in a single tube (one-tube nested real-time PCR), was developed for detecting M. tuberculosis, which takes advantage of two PCR techniques, i.e., nested PCR and real-time PCR. One-tube nested real-time PCR was designed to have two sequential reactions with two sets of primers and dual probes for the insertion sequence (IS) 6110 sequence of M. tuberculosis in a single closed tube. The minimum limits of detection of IS6110 real-time PCR and IS6110 one-tube nested real-time PCR were 100 fg/μL and 1 fg/μL of M. tuberculosis DNA, respectively. AdvanSure TB/non-tuberculous mycobacteria (NTM) real-time PCR, IS6110 real-time PCR, and two-tube nested real-time PCR showed 100% sensitivity and 100% specificity for clinical M. tuberculosis isolates and NTM isolates. In comparison, the sensitivities of AdvanSure TB/NTM real-time PCR, single IS6110 real-time PCR, and one-tube nested real-time PCR were 91% (152/167), 94.6% (158/167), and 100% (167/167) for sputum specimens, respectively. In conclusion, IS6110 one-tube nested real-time PCR is useful for detecting M. tuberculosis due to its high sensitivity and simple manipulation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 299~303 | - |
dc.relation.isPartOf | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Limit of Detection | - |
dc.subject.MESH | Mycobacterium tuberculosis/genetics* | - |
dc.subject.MESH | Mycobacterium tuberculosis/isolation & purification | - |
dc.subject.MESH | Nontuberculous Mycobacteria/genetics | - |
dc.subject.MESH | Polymerase Chain Reaction/instrumentation | - |
dc.subject.MESH | Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction/instrumentation | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction/methods* | - |
dc.subject.MESH | Real-Time Polymerase Chain Reaction/standards | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Sputum/microbiology | - |
dc.subject.MESH | Tuberculosis/diagnosis | - |
dc.subject.MESH | Tuberculosis/microbiology* | - |
dc.title | Development of a highly sensitive one-tube nested real-time PCR for detecting Mycobacterium tuberculosis. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Yeonim Choi | - |
dc.contributor.googleauthor | Bo Young Jeon | - |
dc.contributor.googleauthor | Tae Sun Shim | - |
dc.contributor.googleauthor | Hyunwoo Jin | - |
dc.contributor.googleauthor | Sang Nae Cho | - |
dc.contributor.googleauthor | Hyeyoung Lee | - |
dc.identifier.doi | 10.1016/j.diagmicrobio.2014.08.009 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J00731 | - |
dc.identifier.eissn | 1879-0070 | - |
dc.identifier.pmid | 25241639 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0732889314003502 | - |
dc.subject.keyword | IS6110 | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.subject.keyword | One-tube nested real-time PCR | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 80 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 299 | - |
dc.citation.endPage | 303 | - |
dc.identifier.bibliographicCitation | DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.80(4) : 299-303, 2014 | - |
dc.identifier.rimsid | 38429 | - |
dc.type.rims | ART | - |
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