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Pax6 expressed in osteocytes inhibits canonical wnt signaling

Title
 Pax6 expressed in osteocytes inhibits canonical wnt signaling 
Other Titles
 Osteocyte에서 발현하는 Pax6에 의한 canonical wnt signaling 억제 
Issue Date
2013
Publisher
 Graduate School, Yonsei University 
Description
Dept. of Medical Science/박사
Abstract
Pax6 is a transcription factor, which belongs to the paired box-containing gene family. It regulates developmental processes, especially in the eyes, central nervous tissues and craniofacial structures. However, the role of Pax6 in bone has never been studied. This study report that Pax6 is expressed at both the mRNA and protein level in the calvaria and long bones of adult mice as well as osteocyte-like MLOY4 cells and suppresses the canonical Wnt signaling pathway. Moreover, the expression levels of Pax6 were much higher in the calvaria than the long bones, and Pax6 was also expressed at E16 to E18 in both the calvaria and long bones. Knockdown of Pax6 in MLOY4 cells did not affect cell proliferation or survival; however, the expression of Sost, a late osteocyte marker gene, was significantly decreased. In addition, the overexpression of Pax6 suppressed the canonical Wnt signaling pathway by enhancing the expression of Sost. Furthermore, also demonstrated that Pax6 binds to the Sost promoter and that stimulation of Sost transcription by Pax6 was dependent on a specific Pax6-binding sequence within the promoter. In conclusion, the results of the present study suggest that Pax6 is expressed in bone and may play an important role in osteocyte differentiation by controlling canonical Wnt signaling.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/134548
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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