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Comparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth

Other Titles
 유치와 영구치 치주인대 조직의 유전자 발현에 대한 비교 
Authors
 황동환 
Issue Date
2012
Description
Dept. of Dentistry/박사
Abstract
Periodontal ligament (PDL) tissues play a major role in determining the relationship between deciduous and permanent teeth, and the tissues that surround them. The PDL tissues of deciduous teeth differ from those of permanent teeth in various ways, including in the prevalence and frequency of root resorption. There have been few reports comparing normal deciduous and permanent PDL tissues by DNA microarray analysis. This study compared the gene expression patterns of human deciduous and permanent PDL tissues. PDL samples were obtained from permanent premolars (n=38; from 4 males and 10 females, aged 10–19 years) and anterior deciduous teeth (n=31; from 14 males and 12 females aged 5–13 years) from 40 healthy persons. Several differences in gene expression were revealed between deciduous and permanent PDL tissues by comparative cDNA microarray analysis, reverse-transcription–polymerase chain reaction (RT-PCR), and immunohistochemistry. Out of 28,869 genes, the expressions of 51 (0.177%) were up-regulated by twofold or more; 21 were up-regulated in deciduous PDL tissues and 30 were up-regulated in permanent PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in tissue development, such as LAMC2 (encoding laminin subunit γ-2), COMP (encoding cartilage oligomeric matrix protein), and LAMB3 (encoding laminin subunit β-3), controlling genes such as IGF2BP1 (encoding insulin-like growth factor 2 mRNA-binding protein 1), and genes implicated in chemotaxis, such as CCL21 [encoding chemokine (C-C motif) ligand 21] and CCL18 [encoding chemokine (C-C motif) ligand 18]. The up-regulated genes in permanent PDL tissues were genes related to acute inflammation like IL6 (encoding interleukin 6), neurological responses, such as FOS (encoding c-Fos), FOSB (encoding FBJ murine osteosarcoma viral oncogene homolog B), DOCK3 (encoding dedicator of cytokinesis 3), and LRRTM1 (encoding leucine-rich repeat transmembrane neuronal protein 1), and the control of tooth development, such as ADAMTSL3 (encoding ADAMTS-like 3), and ADAMTS18 (encoding ADAMTS type 1 motif, 18). The data obtained through RT-PCR analysis and immunohistochemistry verified those obtained through cDNA microarray analysis. An understanding of the differences between deciduous and permanent PDL tissues will ultimately provide a comprehensive understanding of the relationship between teeth and the tissues that surround them, from the molecular level (including gene analysis) up to clinical applications
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/134395
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2. 학위논문 > 5. Others > 기타 학위논문
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