Expression of cytokine with asthma related allergens in human gingival epithelial cells
사람구강상피세포에서 천식연관 알러르겐에 의한 시토카인의 발현
Dept. of Dental Science/박사
Asthma is a chronic inflammatory disease of the airway characterized by variable airflow obstruction and bronchial hyper-responsiveness. In addition, patients with asthma have been reported that high prevalence of dental caries and gingivitis, which are induced by low salivation due to the medication for asthma. However, it is not known that asthma related allergens such as house dust mite (HDM) and German cockroach extract (GCE) have direct effects on the generation of gingival inflammation. In the present study, I investigated the level of interlukin-8 (IL-8) mRNA expression and the characteristics of Ca2+ signal by GCE and HDM in the human gingival epithelial cells.HDM and GCE induced increases in IL-8 mRNA expression level and intracellular concentration of Ca2+ ([Ca2+]i), respectively. Endotoxin-free GCE activated protease-activated receptor (PAR) type2, but endotoxin-free HDM did not activate any PARs. In human gingival epithelial cells, whereas lipopolisacaride, a Gram-negative endotoxin, did not induce Ca2+ signal, HDM and GCE containing endotoxins induced Ca2+ signal from thapsigargin (Tg), an inhibitor of sarco/endoplasmic reticulum Ca2+ ATPase, -sensitive Ca2+ stores via phospholipase C (PLC)/inositol 1, 4, 5-trisphosphate (IP3) pathway. These results suggest that asthma related allergens induce Ca2+ signaling and cytokine release in human gingival epithelial cells.