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Characterization of signaling pathways for immune response induced by a recombinant lipoprotein, IlpA of Vibrio vulnificus

Title
 Characterization of signaling pathways for immune response induced by a recombinant lipoprotein, IlpA of Vibrio vulnificus
Other Titles
 패혈증비브리오균 재조합 지질단백질 IlpA 유도 면역반응을 구성하는 면역세포 신호전달체계 규명
Issue Date
2011
Publisher
 Graduate School, Yonsei University
Description
Dept. of Medical Science/박사
Abstract
Vibrio vulnificus is a pathogenic bacterium that causes primary septicemia, which follows a classical septic shock pathway including an overwhelming inflammatory cytokine response. V. vulnificus IlpA is a potent immunogenic lipoprotein that triggers cytokine production in human monocytes by activating the toll-like receptor 2 (TLR2). In this study, we further defined the IlpA signaling pathways involved in cytokine production in the human monocytic cell line, THP-1. TLR2 was involved in cytokine production by complexing with TLR1, but not TLR6. MyD88 was necessary for IlpA-induced cytokine expression through TLR1/2. Three mitogen activated protein kinases (MAPKs), p38, ERK1/2, and JNK, were activated in THP-1 cells stimulated with recombinant IlpA (rIlpA). Selective inhibition of each MAPK resulted in significant decrease of rIlpA-induced cytokine production. Especially, functional TLR2 was necessary for IlpA-induced activation of p38 and JNK. IlpA augmented the DNA-binding activity of nuclear factor-kappaB (NF-B) and activator protein-1 (AP-1) transcriptional factors to their recognition sites in THP-1 cells. These results suggest that serial activation of TLR1/TLR2, MyD88, the three MAPKs, and NF-B/AP-1 comprises the signaling pathways responsible for proinflammatory cytokine production by V. vulnificus IlpA.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/134048
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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