Cited 0 times in

Sensitivity enhancement and application of surface plasmon resonance biosensors using nanostructures and nanoparticles

Title
Sensitivity enhancement and application of surface plasmon resonance biosensors using nanostructures and nanoparticles
Issue Date
2012
Publisher
Graduate School, Yonsei University
Description
Program for Nanomedical Science and Technology/박사
Abstract
Surface plasmon resonance (SPR) has been applied to biosensing applications due to its intriguing characteristics arising from surface-selective detection of optical indices. Among researches in this field, enhancing sensitivity has received much interest in terms of application of SPR biosensors to molecular detection of DNAs and proteins. Conventional SPR biosensor can detect 1 pg/mm 2 of typical biochemical molecules, which corresponds to 6 × 10 7 molecules per square millimeter, assuming small molecules with 10 kDa. Thus, enhancing sensitivity and subsequently improving the detection limit is the key to achieving inexpensive and efficient molecular detection apparatus.This study suggests a hybrid mode for sensitivity enhancement scheme combining plasmonic enhancement via nanoparticles and localized surface plasmon effect obtained with surface relief nanostructure. Namely, use of gold nanoparticles (GNPs) and gold@silica core-shell nanoparticles (CSNPs) served as index contrast, while employment of localized surface plasmon (LSP) nanograting contributed to boost signal per unit index change. The hybrid sensing scheme demonstrated 18 to 36 times enhanced detection sensitivity for DNA hybridization monitoring, respectively for GNPs and CSNP conjugation.Enhanced sensitivity suggests capability of femto-scale measurements of biomolecular interactions on LSP nanostructures, and potential application to high throughput screening in drug discovery and medical researches promises retrenchment of vast cost.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/133747
Appears in Collections:
2. 학위논문 > 5. Others > 기타 학위논문
Yonsei Authors
사서에게 알리기
  feedback
Fulltext
교내이용자 서비스로 제공됩니다.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse