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Chitinase-like protein YKL-40 regulates hyperoxia-induced apoptosis in human airway epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 김미나 | - |
dc.date.accessioned | 2015-12-24T08:33:47Z | - |
dc.date.available | 2015-12-24T08:33:47Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/133695 | - |
dc.description | Dept. of Medical Science/석사 | - |
dc.description.abstract | YKL-40 is chitinase-like protein that lacks chitinase activity. Prolonged exposure to 100% oxygen causes hyperoxic acute lung injury characterized by alveolar epithelial cell injury and death. We investigated the role of YKL-40 regulating hyperoxia-induced apoptosis in human airway epithelial cells. Human airway epithelial cell line, BEAS-2B, was exposed to > 93% oxygen for 24-72 hours. Hyperoxia induced apoptosis was confirmed by flow cytometry with Annexin-V and PI staining. The mRNA and protein expression of YKL-40, caspase 3 and caspase 7 was determined by real time PCR and Western blotting. YKL-40 short hairpin RNA (shRNA) and over-expression vectors were transfected to cells to examine the requirement of YKL-40. Hyperoxia increased FITC-Annexin V positive cells compared with room air. Caspase 3 and 7 , representative apoptosis regulators, were increased by hyperoxia on mRNA and protein level. YKL-40 expression was also increased. YKL-40 shRNA transfected cells expressed lower level of caspase 3 and 7 mRNA and protein than untransfected cells after exposing to hyperoxia, whereas YKL-40 over-expression vector transfected cells showed higher level of caspase 3 and 7 than untransfected cells. We concluded that hyperoxia induced apoptosis associates with YKL-40 expression and that YKL-40 could be considered as one of apoptosis regulators in oxidative stressed human airway epithelial cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.publisher | Graduate School, Yonsei University | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Chitinase-like protein YKL-40 regulates hyperoxia-induced apoptosis in human airway epithelial cells | - |
dc.title.alternative | 고농도 산소에 의한 인체 기도상피세포 손상에서 YKL-40 의 apoptosis 조절 | - |
dc.type | Thesis | - |
dc.contributor.alternativeName | Kim, Mi Na | - |
dc.type.local | Thesis | - |
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