Cited 0 times in

First in human evaluation of a newly developed integrin binding positron emission tomography tracer, 18F-RGD-K5 : comparison with 18F-FDG uptake pattern and microvessel density in patients with breast cancer

Title
First in human evaluation of a newly developed integrin binding positron emission tomography tracer, 18F-RGD-K5 : comparison with 18F-FDG uptake pattern and microvessel density in patients with breast cancer
Other Titles
유방암 환자에서 새로운 integrin 결합 양전자방출단층촬영 추적자, 18F-RGD-K5의 최초 임상 시험: 18F-FDG 섭취 및 미세혈관농도와의 비교
Issue Date
2011
Publisher
Graduate School, Yonsei University
Description
Dept. of Medicine/석사
Abstract
The purpose of this study was to evaluate the biodistribution and dosimetry of a newly developed integrin binding agent, 18F-RGD-K5, in healthy volunteers, and to compare the uptake pattern of 18F-RGD-K5 with 18F-FDG and microvessel density (MVD) in tumor specimen in patients with breast cancer. Methods: Four female healthy volunteers underwent five successive dynamic 18F-RGD-K5 PET scans and radiation absorbed doses were calculated. Eleven patients with primary or metastatic breast cancer underwent 18F-FDG and 18F-RGD-K5 PET scans. Biopsy specimen was obtained in 9 patients and immunohistochemistry using a mouse monoclonal antibody against CD31 was performed. In vitro 18F-FDG and 18F-RGD-K5 uptake and western blot analysis in breast cancer cell lines MCF-7 and MDA-MB-231 were also examined. Results: The radiation dose from 18F-RGD-K5 is similar to the dose from 18F-FDG and other 18F-based imaging agents. In 11 patients, a total of 189 lesions were identified on 18F-FDG PET scan, and 166 lesions (87.8%) showed increased 18F-RGD-K5 uptake. In comparison with 18F-RGD-K5 uptake, 18F-FDG uptake was higher in 108 lesions, and similar in 56 lesions. In 2 lesions, however, 18F-RGD-K5 uptake was higher. Although statistically insignificant, a weak correlation was observed between SUVs of 18F-RGD-K5 and MVD (r=0.43; P=0.25). No correlation was found between SUVs of 18F-RGD-K5 and 18F-FDG, or microvessel area. In vitro experiments showed that integrin β3 protein was more highly expressed in more aggressive MDA-MB-231 cells than in MCF-7 cells. However, 18F-RGD-K5 uptake was higher in MCF-7 cells than in MDA-MB-231 cells. These data suggest that 18F-RGD-K5 might recognize not only integrin αvβ3 but also other integrin subtypes, or a subpopulation of integrin αvβ3. Conclusion: Although 18F-RGD-K5 uptake was not well correlated with MVD, it is a safe and useful marker for noninvasive visualization of integrin expression in patients with breast cancer.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/133632
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 석사
Yonsei Authors
사서에게 알리기
  feedback
Fulltext
교내이용자 서비스로 제공됩니다.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse