Sulfur 및 lithium이 家兎 血中 酒精濃度에 미치는 影響에 관한 實驗的 硏究

Abstract

[한글]

Effects of Sulfur and Lithium on Blood Alcohol Level in Rabbits

Sung Kil Min

Department of Medical Science, Graduate School, Yonsei University

In recent year, it has been known that some simple elements have marked effects

as a psychotropic drug. Lithium among others, is now considered to be an extremely

promising and intriguing drug in the treatment of mania and other psychotic

excitements. Sulfur, which is also an element, has been known as an important

component of enzymes and other organic substances. It was once need in the

pyretotherapy of dementia praecox and general paresis. And sulfur was known to

cause some calming effects, drowsiness, narcosis and central motor depression.

It has been lately reported that chlorpremazine, lithium ion, and several other

psychotropic drugs elevated the blood alcohol level in rabbits. In view of these

findings, the author conducted an animal experiment to investigate the effects of

sulfur, or in combination with lithium ion, on blood alcohol level in rabbits.

Material and Method

1. Experimental work was done with mature rabbits of both sexes, weighing about

2.0kg.

2. The experimental animals were divided into two groups; control and

experimental group, control group was given alcohol only, and experimental group

was divided into three groups; alcohol+sulfur group, alcohol+lithium group and

alcohol+lithium+sulfur group.

3. Sublimed sulfur capsule was given orally. alcohol+sulfur group and

alcohol+lithium+sulfur group were divided into three subgroups. To the 1st subgroup

sulfur was given 100mg/kg of body weight daily for 5 days, to the 2nd subgroup,

100mg/kg of body weight daily for 10 days, and to the 3rd subgroup, 200mg/kg of

body weight daily for 5 days. The last dose of sulfur was given 1.5 hour before

alcohol administration.

4. Lithium chloride solution, 6.36%, was given in a dose of 3.0mEq/kg of body

weight daily for 4 days by intravenous route.

The last dose was given 1 hour before alcohol administration.

5. In all groups, 20 vol. % ethanol solution was given in a dose of 5.0ml/kg of

body weight in 5 minutes by intravenous route.

6. All of the blood specimens were obtained by cardiac puncture at 15 and 45

minutes after alcohol administration.

7. the blood alcohol level was determined by Cavett's method.

Results

1. Alcohol+sulfur group.

In 1st subgroup, sulfur elevated the blood alcohol level at 15 minutes and

lowered at 45 minutes after alcohol administration. but those results were not

statistically significant. (p>0.05 in both) In both 2nd and 3rd subgroups, sulfur

elevated the blood alcohol level significantly at 15 minutes after alcohol

administration, (P<0.01) but not at 45 minutes.(P>0.05)

2. Alcohol+lithium group

Lithium ion elevated the blood alcohol level significantly at both 15 and 45

minutes after alcohol administration. (P<0.01 and P<0.05 respectively)

3. alcohol+lithium+sulfur group.

In all subgroups, sulfur combined with lithium ion elevated significantly the

blood alcohol level at both 15 and 45 minutes after alcohol administration.

(P<0.01) The blood alcohol levels of these subgroups were significantly higher than

those of all subgroups of alcohol+sulfur group and alcohol+lithium group.(P<0.01)

Conclusions

1. The orally administrated sublimed sulfur in a dose of 100 mg/kg/day for 5 days

had no significant effect on blood alcohol level in rabbits. But sulfur in a dose

of 100 mg/kg/day for 10 days and in a dose of 200 mg/kg/day for 5 days elevated

significantly the blood alcohol level in rabbits at 15 minutes, but not at 45

minutes after alcohol administration.

2. The intravenous injection of lithium chloride in a dose of 3 mEq/kg/day for 4

days elevated significantly the blood alcohol level in rabbits at both 15 and 45

minutes after alcohol administration.

3. The sulfur combined with lithium chloride elevated significantly the blood

alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.

[영문]

In recent year, it has been known that some simple elements have marked effects as a psychotropic drug. Lithium among others, is now considered to be an extremely promising and intriguing drug in the treatment of mania and other psychotic excitements. Sulfur, which is also an element, has been known as an important

component of enzymes and other organic substances. It was once need in the pyretotherapy of dementia praecox and general paresis. And sulfur was known to cause some calming effects, drowsiness, narcosis and central motor depression.

It has been lately reported that chlorpremazine, lithium ion, and several other psychotropic drugs elevated the blood alcohol level in rabbits. In view of these findings, the author conducted an animal experiment to investigate the effects of sulfur, or in combination with lithium ion, on blood alcohol level in rabbits.

Material and Method

1. Experimental work was done with mature rabbits of both sexes, weighing about 2.0kg.

2. The experimental animals were divided into two groups; control and experimental group, control group was given alcohol only, and experimental group was divided into three groups; alcohol+sulfur group, alcohol+lithium group and alcohol+lithium+sulfur group.

3. Sublimed sulfur capsule was given orally. alcohol+sulfur group and alcohol+lithium+sulfur group were divided into three subgroups. To the 1st subgroup sulfur was given 100mg/kg of body weight daily for 5 days, to the 2nd subgroup, 100mg/kg of body weight daily for 10 days, and to the 3rd subgroup, 200mg/kg of

body weight daily for 5 days. The last dose of sulfur was given 1.5 hour before alcohol administration.

4. Lithium chloride solution, 6.36%, was given in a dose of 3.0mEq/kg of body

weight daily for 4 days by intravenous route.

The last dose was given 1 hour before alcohol administration.

5. In all groups, 20 vol. % ethanol solution was given in a dose of 5.0ml/kg of body weight in 5 minutes by intravenous route.

6. All of the blood specimens were obtained by cardiac puncture at 15 and 45 minutes after alcohol administration.

7. the blood alcohol level was determined by Cavett's method.

Results

1. Alcohol+sulfur group.

In 1st subgroup, sulfur elevated the blood alcohol level at 15 minutes and lowered at 45 minutes after alcohol administration. but those results were not statistically significant. (p>0.05 in both) In both 2nd and 3rd subgroups, sulfur elevated the blood alcohol level significantly at 15 minutes after alcohol administration, (P<0.01) but not at 45 minutes.(P>0.05)

2. Alcohol+lithium group

Lithium ion elevated the blood alcohol level significantly at both 15 and 45 minutes after alcohol administration. (P<0.01 and P<0.05 respectively)

3. alcohol+lithium+sulfur group.

In all subgroups, sulfur combined with lithium ion elevated significantly the blood alcohol level at both 15 and 45 minutes after alcohol administration.

(P<0.01) The blood alcohol levels of these subgroups were significantly higher than those of all subgroups of alcohol+sulfur group and alcohol+lithium group.(P<0.01)

Conclusions

1. The orally administrated sublimed sulfur in a dose of 100 mg/kg/day for 5 days had no significant effect on blood alcohol level in rabbits. But sulfur in a dose of 100 mg/kg/day for 10 days and in a dose of 200 mg/kg/day for 5 days elevated

significantly the blood alcohol level in rabbits at 15 minutes, but not at 45 minutes after alcohol administration.

2. The intravenous injection of lithium chloride in a dose of 3 mEq/kg/day for 4 days elevated significantly the blood alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.

3. The sulfur combined with lithium chloride elevated significantly the blood alcohol level in rabbits at both 15 and 45 minutes after alcohol administration.