Studies with Co57-Vitamin B12 on metabolism of vitamin B12 liver diseases
In 1955 Jerzy Glass et al. reported the pattern of uptake of vitamin B12 in liver, spleen and bone marrow using Co**60 -vitamin B12. In 1958 they observed that the uptake of vitamin B12 in liver was lower in patients with liver diseases than in normal persons. Stein et al. confirmed the decreased uptake in animal
The purpose of this study was to estimate the degree of the uptake of the vitamin B12 in the liver in normal subjects and in patients with liver diseases.
One uCi. of Co**57 -vitamin B12 was injested to normal subjects and patients with liver cirrhosis and with acute hepatitis, and checked serum activity, stool count and surface counts on liver, spleen and iliac crest.
The following results were obtained:
1. Serum counts were highest at 8 hours after ingestion of 1 uCi. of Co**57 -vitamin B12 orally, but there was no difference between in normal subjects and in patients with liver disease.
2. The amount of Co**57 -vitamin B12 excreted in stool was higher in patients with liver diseases than in normal subjects.
3. In normal subjects the surface counts on liver area reached to the peak on the 45h day after ingestion and remained there until 10th day.
4. In patient with liver disease the surface counts on liver area were lower in general than in normal subjects, and decreased from the 4th day and showed a significant difference on the 10th day compared to that of normal subjects.
5. The surface counts on spleen and iliac crest were lower than on liver and were gradually decreased in both groups.