Hypoxia preconditioned adipose tissue derived mesenchymal stem cells improve the survival of mouse neural stem cells in vitro injury models

Abstract

[영문]

Hypoxic stem cells have resistance of hypoxia condition and hypoxic preconditioning can increase their tolerance of hypoxia condition after transplantation into damaged tissue accompanying with ischemia. In order to improve survival of the therapeutic stem cells carrying a reporter DsRed Gene after transplantation into the injured spinal cord, this study examined the effect of a novel strategy that co-culture the mouse neural stem cells expressing DsRed (mNSC-DsRed) with hypoxic preconditioned adipose tissue derived mesenchymal stem cells(HP-MSC). Hypoxia preconditioning MSC that cultured for 1 day in hypoxia condition expressed the surface marker CD90 and CD105 like normal conditioned MSC (N-MSC). It suggest that hypoxia preconditioning not change the nature caracterization of AT-MSC. In the hypoxia and serum starvation injury model, the viability of mNSC-DsRed was clearly increased in by co-culture with HP-MSC or with normoxic conditioned MSC (N-MSC), compared with mNSC-DsRed alone. Espesially HP-MSC showed better protective effect on mNSC-DsRed survival than N-MSC. MSC co-clture system could inhibit the early apoptosis of mNSC compare to control group in serum deprivation and oxidative stress injury. These findings suggest that the strategy of hypoxic preconditioning stem cells and gene delivery platform using stem cells may represent a more effective stem cell therapy.