Guinea Pig 성장에 따른 비점막의 Lectin 결합반응 양상

Abstract

[한글]

임상적으로 소아와 성인에 있어서 이비인후과 영역에서 가장 흔한 질환 중에 하나인 비염과 부비등염의 유병율이나 임상양상이 다른데 현재까지는 해부학적으로 소아에 있어 상악등 자연구가 비교적 크기 때문이라는 설명이 있으나 성장에 따른 소아의 비점막 기능에

대한 연구는 아직 미흡한 실정이다. 성장에 따라 비점막 당단백질의 분포 및 발현시기가 다르다면, 소아연령층에서 비과적 감염이 잘 되는 원인을 이해하는데 도움이 될 것으로 생각되었다.

이에 연구자는 guinea pig의 비점막에 분포하는 당단백질의 변화를 lectin조직화학법을 이용하여, 출생후 연령 및 부위별로 관찰하여 다음과 같은 경과를 얻었다.

1) Con-A, RCA-I 반응은 생후 1일부터, DBA 반응은 생후 7일부터 모든 호흡 점막에서 관찰되었다.

2) UEA-I, WGA 및 SBA는 생후 1일에는 비강저, 비중격하부 및 상악골갑개 점막 상피에서만 반응을 보였으나 UEA-I과 WGA는 생후 3일부터, SBA는 생후 7일부터 모든 호흡점막에서 관찰되었다.

3) 후각점막애서는 생후 1일부터 PNA, WGA, Con-A의 반응을 관찰할 수 있었으나 생후 성장에 따른 변화는 없었다.

이상의 결과로 보아, 당단백질의 비점막내 발현시기와 부위별 분포의 차이가 guinea pig와 생후 성장에 따라 비점막에 영향을 끼칠 것으로 생각된다.

Lectin binding patterns in nasal mucosa of growing guinea pig

Seong Soo Hong

Department of Medical Science The Graduate School,Yonsei University

(Directed by Professor In Yong Park)

Clinically there are differences in the pathophysiologic patterns of the

infectious diseases of nasal mucosa, especially of rhinitis and sinusitis,

according to growth. But acceptable explanation of such differences is not

established except the anatomical cause such as relative larger size of the natural

ostium of naxillary sinus in childhood. Glycoprotein is the one of the most

important substances for defense mechanism and transmitter of intercellular

information.

We can postulate that if there is differences in distribution of glycoproteins

according to the growth, it might be one of the immunohistochemical clues to

understand the differences of the pathophysiologic pattern. This study is designed

to observe the distributions of glycoproteins in nasal mucosa of guinea pig

according to the age and sites, using lectin immunohistochemistry. The significant

results obtained ape are follows:

1) Con-A and RCA-I binding were noted since birth and DBA was noted from the

seventh day after birth in all respiratory mucosa.

2) UEA-1, WGA and SBA binding were observed at nasal floor. lower septum and

maxilloturbinate since birth. UEA-I and WGA binding were noted from the third day

after birth and SBA binding was noted from the seventh day after birth in

ethmoturbinate and upper septum.

3) PNA, WGA and Con-A binding were noted since birth in olfactory mucosa.

From above results, the patterns of appearance and distribution of glycoproteins

seems to contribute the funotion of the nasal mucosa of growing guinea pig.

[영문]

Clinically there are differences in the pathophysiologic patterns of the infectious diseases of nasal mucosa, especially of rhinitis and sinusitis, according to growth. But acceptable explanation of such differences is not established except the anatomical cause such as relative larger size of the natural

ostium of naxillary sinus in childhood. Glycoprotein is the one of the most important substances for defense mechanism and transmitter of intercellular information.

We can postulate that if there is differences in distribution of glycoproteins according to the growth, it might be one of the immunohistochemical clues to understand the differences of the pathophysiologic pattern. This study is designed to observe the distributions of glycoproteins in nasal mucosa of guinea pig

according to the age and sites, using lectin immunohistochemistry. The significant results obtained ape are follows:

1) Con-A and RCA-I binding were noted since birth and DBA was noted from the seventh day after birth in all respiratory mucosa.

2) UEA-1, WGA and SBA binding were observed at nasal floor. lower septum and maxilloturbinate since birth. UEA-I and WGA binding were noted from the third day after birth and SBA binding was noted from the seventh day after birth in ethmoturbinate and upper septum.

3) PNA, WGA and Con-A binding were noted since birth in olfactory mucosa.

From above results, the patterns of appearance and distribution of glycoproteins seems to contribute the funotion of the nasal mucosa of growing guinea pig.