1. Estrogen관류로 적출취장의 외분비기능은 항진되었으며 estrogen 10**-8 M이상의 농도에서 secretin에 의한 취액분비와 CCK자극에 의한 amylase분비가 증가되었다.
2. Progesterone관류로 취외분비는 증가하여 secretin자극에 의한 취액분비는 10**-6 M관류시 증가하였고, amylase분비는 CCK자극에 의한 progesterone 10**-7 M첨가로 증가하였다.
3. Estrogen 10**-7 M 존재하에 progesterone을 관류함으로써 amylase 분비 변동은 관찰되지 않았으며, secretin자극에 의한 취액유출은 변동이 없었다.
이상의 결과로 미루어 보아 estrogen 및 progesterone등 난소 홀몬은 취선포에 직접 작용하여, 취액 및 취효소분비를 항진시키며 취외분비에 대한 progesterone의 효과는 estrogen과 상관없이 나타난다고 생각한다.
Influence of Ovarian Hormones on the Exocrine Secretion in Isolated Cat Pancreas
Jae Won choi
Department of Medical Science, The Graduate School, Yonsei University
(Directed by Professor Kyung Hwan Kim)
Regulation of pancreatic exocrine secretion is comprised of a complex interplay
between hormonal and nervous systems. It has been claimed that steroid hormones may
participate in maintaining the exocrine function of the pancreas. Corticosteroids
and ovarian hormones are synthesized from the same precursor, cholesterol, in vivo
and they are important regulators of homeostasis.
Though the effects of corticosteroids on pancreatic function are not consistant,
it is widely accepted that the glucocorticoid inhibits the secretory function
whereas mineralocorticoid stimulates.
Several studies have suggested that ovarian hormones exert significant influence
on the exocrine pancreas. And pancreatic function of exocrine cells may be
regulated by estrogen and estrogen-binding protein. Also, it was reported that
protesterone increased both the cellular content of amylase and cytoplasmic mRNA of
rat tumor cell line AR 42J.
In the present study, attempt was made to examine the direct effects of estrogen
and progesterone on exocrine function employing saline perfused isolated cat
pancreas.
Mongrel cats, of both sexes weighing about 1.5Kg , were anesthetized with
secobarbital (30mg/kg, ip), then the pancreas was isolated and perfused with Krebs
Ringer bicarbonate buffer(KRBB) gassed with 95% 0^^2-5% CO^^2 at the rate of 6
ml/min. Ovarian hormone, estrogen or progesterone was added to the perfusate for 30
minutes. Submaximal dose of secretin was infused to maintain pancreatic flow and
cholecystokinin was injected to evoke the enzyme secretion in the presence or
absence of ovarian hormones.
The results are summarized as fellows.
1. Perfusion of estrogen increased the pancreatic flow as well as CCK-stimulated
amylase secretion.
2. CCK-stimulated secretion of amylase was increased by perfusion with
progesterone and over 10**-6 M concentration secretin-stimulated pancreatic flow
was also increased.
3. The effect of progesterone on the exocrine secretory function was not enhanced
or inhibited by background perfusion of estrogen in isolated perfused pancreas.
These results suggest that ovarian hormones exert significant influence on the
exocrine secretory function, both enzyme and waters of the pancreas and this effect
might be exerted by direct effect on the pancreas.
[영문]
Regulation of pancreatic exocrine secretion is comprised of a complex interplay between hormonal and nervous systems. It has been claimed that steroid hormones may participate in maintaining the exocrine function of the pancreas. Corticosteroids and ovarian hormones are synthesized from the same precursor, cholesterol, in vivo and they are important regulators of homeostasis.
Though the effects of corticosteroids on pancreatic function are not consistant, it is widely accepted that the glucocorticoid inhibits the secretory function whereas mineralocorticoid stimulates.
Several studies have suggested that ovarian hormones exert significant influence on the exocrine pancreas. And pancreatic function of exocrine cells may be regulated by estrogen and estrogen-binding protein. Also, it was reported that
protesterone increased both the cellular content of amylase and cytoplasmic mRNA of rat tumor cell line AR 42J.
In the present study, attempt was made to examine the direct effects of estrogen and progesterone on exocrine function employing saline perfused isolated cat pancreas.
Mongrel cats, of both sexes weighing about 1.5Kg , were anesthetized with secobarbital (30mg/kg, ip), then the pancreas was isolated and perfused with Krebs Ringer bicarbonate buffer(KRBB) gassed with 95% 0^^2-5% CO^^2 at the rate of 6
ml/min. Ovarian hormone, estrogen or progesterone was added to the perfusate for 30 minutes. Submaximal dose of secretin was infused to maintain pancreatic flow and cholecystokinin was injected to evoke the enzyme secretion in the presence or
absence of ovarian hormones.
The results are summarized as fellows.
1. Perfusion of estrogen increased the pancreatic flow as well as CCK-stimulated amylase secretion.
2. CCK-stimulated secretion of amylase was increased by perfusion with progesterone and over 10**-6 M concentration secretin-stimulated pancreatic flow was also increased.
3. The effect of progesterone on the exocrine secretory function was not enhanced or inhibited by background perfusion of estrogen in isolated perfused pancreas.
These results suggest that ovarian hormones exert significant influence on the exocrine secretory function, both enzyme and waters of the pancreas and this effect might be exerted by direct effect on the pancreas.