Formocresol, Glutaraldehyde 가 유견 계승치 치배 및 주위조직에 미치는 영향에 관한 병리조직학적 연구

Abstract

[한글]

저자는 formocresol과 glutaraldehyde가 치배 및 주위조직에 미치는 영향을 관찰하기 위하여 치수강저의 치근이개부를 천공시켜 인위적으로 치조골에 손상을 준 후 대조군으로는 생리적 식염수를, 실험군으로는 formocresol과 glutaraldehyde를 사용하였다. 생후 약

8∼ 9주된 체중 2.5kg내외의 유견 4마리를 본 실험에 사용하였고 2시간, 8일, l6일, 24일, 35일 간격으로 나누어 시행하였으며 최종실험후 실험동물을 회생시켜서 적출한 조직은 10% formalin용액에 고정한 다음 5% Nitric acid로 탈회하여 5∼ 7μ두께로 절편을 제작

하고 Hematoxylin-Eosin에 염색한 후 광학현미경으로 검경한 결과 다음과 같은 결론을 얻었다.

1. 대조군에서는 정상적인 치유과정을 보였고 실험16일에 손상부의 상피화가 완성되었음을 관찰하였다. 염증성 반응은 손상부 표면에 국한되었으며 그 외의 골 및 골수조직은 정상 이였고 영구치 치배조직도 정상이였다.

2. Formocresol 도포군과 glutaraldehyde 도포군의 조직반응은 동일하였고 염증성 반응은 대조군에 비해 경미하였으나 표면 상피화는 대조군에 비하여 지연되었다.

3. Formocresol 도포군과 glutaraldehyde 도포군은 표층 골수조직에 괴사를 유발시켰고 실험24일에서 영구치 치배 상부의 골조직 중앙부가 소실되고 결체조직으로 대치되어 손상부 표층의 연조직과 영구치 치배 상부의 연조직이 연결되었으며 잔여골면에서 파골현상이 왕성하였다.

4. Formocresol 도포군과 glutaraldehyde 도포군에서 영구치 치배의 손상은 없었다.

5. Formocresol 도포군과 glutaraldehyde 도포군에서 양측 치근막은 정상이었으나 손상부근처의 치근의 백아질과 상아질의 부분적인 흡수상을 보였다.

THE EFFECTS OF FORMOCRESOL AND GLUTARALDEHYDE ON THE PERFORATED INTERRADICULAR

TISSUES AND TOOTH GERMS OF PRIMARY TEETH IN DOGS

Byung Jai Choi

Department of Dental Science, Graduate School, Yonsei University

(Directed by Associate Professor Jong Gap Lee, D.D.S., M.S.D., Ph.D.)

The purpose of this study is to observe the effect of formocresol and

glutaraldehyde to tooth germs and periapical tissues after perforation of

interradicular portion of pulpal floor and application of physiological saline

solution in control groups, formocresol and glutaraldehyde in experimental groups.

The following results were obtained

1. In control groups, normal healing processes were seen, and, on the sixteenth

day, the epithelization of injured areas was completed. Inflammatory reactions were

limited to the injured surface, and the underlying alveolar bone were normal and

successive tooth germs were normal.

2. In both formocresol groups and glutaraldehyde groups, tissue reactions were

identical. Inflammatory reactions were slightly compared wish control groups, but

the surface epithelizations were delayed compared with control group.

3. In both formocresol and glutaraldehyde groups, necrosis was seen in

superficial tissue of bone marrow, and at 24th day, center area of bone marrow on

the successive tooth germs were losed and replaced with connective tissue, and

superficial soft tissue of the injured area was connected with soft tissue on the

successive tooth germ. In remaining alveolar bone, osteoclastic reaction was

remarkable.

4. In both formocresol and glutaraldehyde groups, there is no injury to the

successive tooth germs.

5. In both formocresol and glutaraldehyde groups, periodontal membrane was

normal, but the partial resorption of cementum and dentin near the injured area

were seen.

[영문]

The purpose of this study is to observe the effect of formocresol and glutaraldehyde to tooth germs and periapical tissues after perforation of interradicular portion of pulpal floor and application of physiological saline solution in control groups, formocresol and glutaraldehyde in experimental groups.

The following results were obtained

1. In control groups, normal healing processes were seen, and, on the sixteenth day, the epithelization of injured areas was completed. Inflammatory reactions were limited to the injured surface, and the underlying alveolar bone were normal and

successive tooth germs were normal.

2. In both formocresol groups and glutaraldehyde groups, tissue reactions were identical. Inflammatory reactions were slightly compared wish control groups, but the surface epithelizations were delayed compared with control group.

3. In both formocresol and glutaraldehyde groups, necrosis was seen in superficial tissue of bone marrow, and at 24th day, center area of bone marrow on the successive tooth germs were losed and replaced with connective tissue, and superficial soft tissue of the injured area was connected with soft tissue on the

successive tooth germ. In remaining alveolar bone, osteoclastic reaction was remarkable.

4. In both formocresol and glutaraldehyde groups, there is no injury to the successive tooth germs.

5. In both formocresol and glutaraldehyde groups, periodontal membrane was normal, but the partial resorption of cementum and dentin near the injured area were seen.