실험적 구루병에서 백서치아조직 변화에 관한 현미경학적 연구

Abstract

[한글]

본 논문은 비타민D와 칼슘 및 인이 치아와 그 주위조직의 형성 및 석회화에 미치는 영향을 조사하여 구루병시 나타나는 상아질과 범랑질 및 백아질의 형태학적인 변화를 관찰하고 이것이 치아의 조기상실을 야기하는 치수병변 및 치근단농양을 일으킬 수 있는 가능성을 알기 위한 것이다

생후 24일된 체중 60gm 내외의 백서(白鼠) 64마리를 대조군과 실험군에 각각 24마리와 40마리씩 배정하여 사용하였다. 실험군에서는 Steenbock과 Black의 rachitogenic diet No.2965를 투여하고 대조군에는 시판 혼합사료를 1∼56일 동안 투여한 다음, 매주 대조군 3마리와 실험군 5마리씩을 희생시켜 하악골과 대퇴골을 적출하였으며. 적출한 조직은 10% 중성 formalin 용액에 고정한 후 5% 개미산(formic acid)으로 탈회하고 paraffin에 포매한 다음 6μ두께로 절편을 만들어 hematoxylin과 eosin에 염색한 후 광학현미경으로 검색 결과 다음과 같은 성적을 얻었다.

1. 법랑질의 형성과 석회화는 정상이었으며 형성부전의 형택학적 변화를 보이지 않았다.

2. 실험군에서 상아질의 형성과 석회화는 지연되어 상아질에 대한 predentin의 비율이 대조군에 비하여 현저히 증가되었다.

3. 새로 형성되는 상아질은 대단히 globular하며 predentin과 석회화된 상아질의 경계가 불규칙하였으며 특히 기저부에서는 상아질이 두터우면서 불규칙적인 파상으로 관찰되었다.

4. 백아질의 기질은 형성되나 석회화가 지연되어 cementoid tissue가 증가되었다.

5. 치조골도 기질은 형성되나 석회화가 지연되어 육주골이 유골조직으로 구성되어 두꺼워졌다.

A MICROSCOPIC STUDY OF THE EFFECTS OF EXPERIMENTAL RICKETS ON THE DENTAL STRUCTURE

OF RATS

Don Ok Choi, D.D.S.

Department of Dental Science, Graduate School, Yonsei University

(Directed by Associate Prof. Jong Gap Lee, D.D.S., M.S.D., Ph.D.)

Rickets is not the deposite of minerals in the skeletal tissue and the

retardation of skeletal growth in growing in growing animals.

This study was undertaken to investigate the histologic effects of experimental

rickets on the dental structure of the albino rats, and to show the relationship

between the histological effects and the pulpal disease which induced premature

loss of the primary teeth.

This study was based on material obtained from 40 white rats that were placed on

a rachitogenic diet for a period 1 to 56 days after weaning(at 24days).

In addition, a study was mode of 25 litter mates, 24 to 80days, that were fed a

normal diet.

The following results were obtained:

1. Enamel formation and calcification showed no significant changes and no

hypoplasia.

2. Dentin formation and calcification was retarded and disturbed. In the

experimental group, predentin/calcified dentin was remarkablly increased.

3. Newly formed dentin showed interglobular texture (less homogenous

calcificaton) and the predentin was significantly wider and thicker, and there was

an irregular wave in the basal portion of the rat's incisors.

4. In cementum, Matrix formed at almost a normal rate but calcification was

defective.

So cementoid tissue was increasead.

5. The formation of the alveolar bone was at almost a normal rate but

calcification was retarded. The trabecular bone was filled with osteoid tissue and

thicker than in normal groups.

[영문]

Rickets is not the deposite of minerals in the skeletal tissue and the retardation of skeletal growth in growing in growing animals.

This study was undertaken to investigate the histologic effects of experimental rickets on the dental structure of the albino rats, and to show the relationship between the histological effects and the pulpal disease which induced premature loss of the primary teeth.

This study was based on material obtained from 40 white rats that were placed on a rachitogenic diet for a period 1 to 56 days after weaning(at 24days).

In addition, a study was mode of 25 litter mates, 24 to 80days, that were fed a normal diet.

The following results were obtained:

1. Enamel formation and calcification showed no significant changes and no hypoplasia.

2. Dentin formation and calcification was retarded and disturbed. In the experimental group, predentin/calcified dentin was remarkablly increased.

3. Newly formed dentin showed interglobular texture (less homogenous calcificaton) and the predentin was significantly wider and thicker, and there was an irregular wave in the basal portion of the rat's incisors.

4. In cementum, Matrix formed at almost a normal rate but calcification was defective.

So cementoid tissue was increasead.

5. The formation of the alveolar bone was at almost a normal rate but calcification was retarded. The trabecular bone was filled with osteoid tissue and thicker than in normal groups.