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자궁 Catecholamine에 관한 연구

Title
 자궁 Catecholamine에 관한 연구
Other Titles
 Studies on uterine catecholamines
Issue Date
1964
Publisher
 연세대학교 대학원
Description
의학과/박사
Abstract
[한글] Studies on Uterine Catecholamines Keung Sup Cha Department of Pharmacology and Toxicology Yonsei University College of Medicine, Seoul, Korea (Director: Prof. W.C. Lee, M.D.) For many decades the nerve terminals of the postganglionic adrenergic fibers of the sympathetic nervous system were regarded as a structure to release norepinephrine or epinephrine on the arrival of a nerve impluse. The nature of the transmitter substance is a matter of controversy for a long time, but it is now generally known that norepinephrine would be the transmitter of adrenergic nervous system. Recently a great interest has been centered on the studies of tissue catecholamines and our knowledge on their physiological significance has been greatly advanced. The uterus receives adrenergic terminals from the mesenteric ganglia and considerable large amount of catecholamines have been shown to be contained in this organ. However, the activity of epinephrine, norepinephrine or adrenergic nerve on uterine motility is so complicated that many controversial results have been reported. The present experiments were undertaken to reinvestigate the response of catecholamine to the uterus and to determine the catecholamine content depending on the species and state of estrus. 1. Experiments on the isolated non-pregnant uterus ,of rabbits. A uterine segment of non-pregnant rabbit was suspended in constant temperature bath (38℃) containing 100 ml of Locke's solution aerated with 95% oxygen and 5% carbon dioxide. Spontaneous motility was recorded with an isotonic lever. Addition of epinephrine (5x**-7 M) significantly elevated the tonus and stimulated the spontaneous motility. Pretreatment with dichloroisoproterenol(3×10**-6 M) an adrenergic β-receptor blockade, appears to enhance the stimulatory activity of epinephrine on the isolated non-pregnant uterine segrent. On the other hand, the pretreatment with dibenamine(10**-6M), an adrenergic u-receptor blockade, rendered the uterine muscle to exhibit inhibition after the addition of epinephrine. Following the treatment with both DCI and dibenamine, epinephrine produced no appreciable effects on the spontaneous motility of the uterus. These results suggest that there exist both α and β adrenergic receptors in the uterine muscle and the response to epinephrine of the former is predominant over that of the latter in the non-pregnant uterus of rabbits. 2. Experimental on the isolated pregnant uterus of rabbits. Uterine strips were prepared from placental site and interplacental site of the pregnant uterus (8-28 days) of rabbits respectively and the effects of epinephrine were determined on these isolated uterine strips. Epinephrine produced both stimulatory and inhibitory action on the isolated strips of pregnant rabbit uterus. The former was predominant on the interplacental segment and the latter on the placental segment DCT enhanced the stimulatory activity and dibenamine enhanced the inhibitory action of epinephrine. The pretreatment with both DCI and dibenamine blocked completely the activity of epinephrine. These results indicate that the response to epinephrine of adrenergic β-receptor of uterus increases during pregnancy. 3. Uterine catecholamine concentration. Relative to other organs, the epinephrine content is high in uterus; 35.7% of total catecholamines in rabbit uterus, and 57-69%in human uterus. The concentration of epinephrine in the human uterus was actually greater than that of norepinephrine and it was significantly greater during the proliferative phase of the cycle. The epinephrine concentration was markedly reduced during pregnancy. In the pregnant uterus of rabbits (9-10 days) norepinephrine content markedly reduced (33-fold) and epinephrine was about one half that of the control rabbits at the placental site. The norepinephrine content was also lower at interplacental site while epinephrine content was not statistically different from the control value. In the human pregnant uterus, epinephrine concentration was reduced about 45 percent after 6-8 weeks of ectopic pregnancy, the norepinephrine values were not significantly decreased. At full term and during active labor, the concentrations of both amines at placental sites were less than those found in the non-pregnant group. The concentration of epinephrine in lower uterine segment remained unchanged while that of nor-epinephrine decreased. Of interest was the finding that the norepinephrine concentration of uteri from toxemic patients was two and half times that of lower uterine segment of the non-toxic pregrant individuals. Also the epinephrine concentration was slightly increased.
[영문] For many decades the nerve terminals of the postganglionic adrenergic fibers of the sympathetic nervous system were regarded as a structure to release norepinephrine or epinephrine on the arrival of a nerve impluse. The nature of the transmitter substance is a matter of controversy for a long time, but it is now generally known that norepinephrine would be the transmitter of adrenergic nervous system. Recently a great interest has been centered on the studies of tissue atecholamines and our knowledge on their physiological significance has been greatly advanced. The uterus receives adrenergic terminals from the mesenteric ganglia and considerable large amount of catecholamines have been shown to be contained in this organ. However, the activity of epinephrine, norepinephrine or adrenergic nerve on uterine motility is so complicated that many controversial results have been reported. The present experiments were undertaken to reinvestigate the response of catecholamine to the uterus and to determine the catecholamine content depending on the species and state of estrus. 1. Experiments on the isolated non-pregnant uterus ,of rabbits. A uterine segment of non-pregnant rabbit was suspended in constant temperature bath (38℃) containing 100 ml of Locke's solution aerated with 95% oxygen and 5% carbon dioxide. Spontaneous motility was recorded with an isotonic lever. Addition of epinephrine (5x**-7 M) significantly elevated the tonus and stimulated the spontaneous motility. Pretreatment with dichloroisoproterenol(3×10**-6 M) an adrenergic β-receptor blockade, appears to enhance the stimulatory activity of epinephrine on the isolated non-pregnant uterine segrent. On the other hand, the pretreatment with dibenamine(10**-6M), an adrenergic u-receptor blockade, rendered the uterine muscle to exhibit inhibition after the addition of epinephrine. Following the treatment with both DCI and dibenamine, epinephrine produced no appreciable effects on the spontaneous motility of the uterus. These results suggest that there exist both α and β adrenergic receptors in the uterine muscle and the response to epinephrine of the former is predominant over that of the latter in the non-pregnant uterus of rabbits. 2. Experimental on the isolated pregnant uterus of rabbits. Uterine strips were prepared from placental site and interplacental site of the pregnant uterus (8-28 days) of rabbits respectively and the effects of epinephrine were determined on these isolated uterine strips. Epinephrine produced both stimulatory and inhibitory action on the isolated strips of pregnant rabbit uterus. The former was predominant on the interplacental segment and the latter on the placental segment DCT enhanced the stimulatory activity and dibenamine enhanced the inhibitory action of epinephrine. The pretreatment with both DCI and dibenamine blocked completely the activity of epinephrine. These results indicate that the response to epinephrine of adrenergic β-receptor of uterus increases during pregnancy. 3. Uterine catecholamine concentration. Relative to other organs, the epinephrine content is high in uterus; 35.7% of total catecholamines in rabbit uterus, and 57-69%in human uterus. The concentration of epinephrine in the human uterus was actually greater than that of norepinephrine and it was significantly greater during the proliferative phase of the cycle. The epinephrine concentration was markedly reduced during pregnancy. In the pregnant uterus of rabbits (9-10 days) norepinephrine content markedly reduced (33-fold) and epinephrine was about one half that of the control rabbits at the placental site. The norepinephrine content was also lower at interplacental site while epinephrine content was not statistically different from the control value. In the human pregnant uterus, epinephrine concentration was reduced about 45 percent after 6-8 weeks of ectopic pregnancy, the norepinephrine values were not significantly decreased. At full term and during active labor, the concentrations of both amines at placental sites were less than those found in the non-pregnant group. The concentration of epinephrine in lower uterine segment remained unchanged while that of nor-epinephrine decreased. Of interest was the finding that the norepinephrine concentration of uteri from toxemic patients was two and half times that of lower uterine segment of the non-toxic pregrant individuals. Also the epinephrine concentration was slightly increased.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/117111
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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