흰쥐 기관 및 후두에서 분비조직의 발생

Abstract

[한글]

기관 및 후두의 점막표면은 비강 및 중이나 이관과 마찬가지로 점막섬모 정화작용과, 면역작용, Iysozyme, lactoferrin과 같은 항세균 성분 등의 국소방어작용등, 다양한 점막 방어기전에 의해 보호되고 있는데 이를 분비하는 기관 및 후두의 분비선과 분비세포의

형태에 관한 보고는 많으나 분비활성도와 연관된 분비조직의 발생에 대한 연구는 충분치 못하다.

저자는 흰쥐 기관 및 후두의 분비조직의 발생을 알아보고 향후 기관 및 후두의 발생형 태학적 연구의 기초자료로 삼기 위해서 임신 14일부터 출생 21일 사이의 흰쥐를 이용해서 H & I 염색, AB-PAS 염색방법을 통하여 기관 및 후두 분비조직의 발달을 연구하여 다음

과 같은 결과를 얻었다.

1. 기관 및 후두의 분비조직은 임신 후반기에 발생하며 AB-PAS 염색에서 상피분비세포는 임신 18일에, 점막하 선조직은 출생 1일에 염색되었다.

2. 출생직후 기관 및 후두의 분비세포는 주로 PAS양성 위주의 새포였으나 그 이후 점차적으로 AB-PAS 양성세포가 증가하였다.

3. 발생중인 기관 및 후두의 점막하 선조직은 기관과 인접한 성문하부의 후측방에 가장 많이 분포하였다.

이와같은 결과로 보아 흰쥐 기관 및 후두의 분비조직은 임신 말기에 나타나지만 분비기능은 출생 후 폐의 통기와 함께 활성화되는 것을 알 수 있었다.

Development of Secretory Element in Murine Trachea and Larynx

Jung-Il cho

Department Of Medical Science, The Graduate School, Yonsei University

(Directed by Professor Kwang-Moon Kim)

Mucosal surfaces of the trachea and larynx are protected by several complex

defense mechanism, namely mucociliary clearance, immunereaction, local reaction by

antibacterial secretory products like Iysozyme, lactoferrin, which have also been

demonstrated in the nasal cavity mucosa, the middle ear and eustachian tube mucosa.

The Morphology of secretory glands and cert Is of the trachea and larynx is

well-known, but knowledge concerning their development related to secretory

activity is still sketchy.

The secretory element of the murine trachea and larynx, aging from gestational

day 14 to postnatal day 21, was studied using hematoxylin & eosin and alcian

blue/periodic acid-Schiff staining to investigate the development of secretory

element of the murine trachea and larynx and to provide the basis of morphological

study of the developing trachea and larynx.

The results are as for lows;

1. The epithelial secretory cells were stained with AB-PAS at gestational day 18

and the submucous glands were stained at postnatal day 1, although they started to

develop during the fetal stage.

2, PAS-positive cells were predominant at postnatal day 1, while both AB-and PAS-

positive cells had become numerous since postnatal day 5

3. The submucous gland were most common at posterolateral part of the subglottis,

especialIy at the junction wi th trachea.

The results of this study suggest that the secretory activity starts to be

established immediately after birth with the aeration of the lung.

[영문]

Mucosal surfaces of the trachea and larynx are protected by several complex defense mechanism, namely mucociliary clearance, immunereaction, local reaction by antibacterial secretory products like Iysozyme, lactoferrin, which have also been

demonstrated in the nasal cavity mucosa, the middle ear and eustachian tube mucosa.

The Morphology of secretory glands and cert Is of the trachea and larynx is well-known, but knowledge concerning their development related to secretory activity is still sketchy.

The secretory element of the murine trachea and larynx, aging from gestational day 14 to postnatal day 21, was studied using hematoxylin & eosin and alcian blue/periodic acid-Schiff staining to investigate the development of secretory element of the murine trachea and larynx and to provide the basis of morphological

study of the developing trachea and larynx.

The results are as for lows;

1. The epithelial secretory cells were stained with AB-PAS at gestational day 18 and the submucous glands were stained at postnatal day 1, although they started to develop during the fetal stage.

2, PAS-positive cells were predominant at postnatal day 1, while both AB-and PAS- positive cells had become numerous since postnatal day 5

3. The submucous gland were most common at posterolateral part of the subglottis, especialIy at the junction wi th trachea.

The results of this study suggest that the secretory activity starts to be established immediately after birth with the aeration of the lung.