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몇가지 물질 및 과산화수소수관장이 저산소상태 개의 동맥혈 산소분압에 미치는 영향에 관한 연구

몇가지 물질 및 과산화수소수관장이 저산소상태 개의 동맥혈 산소분압에 미치는 영향에 관한 연구
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Studies on the effect of arterial oxygen partial pressure with the some substances and hydrogenperoxide enema in the hypoxic state
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연세대학교 대학원
[영문] Oliver and Murphy (1920) have attempted to use parenteraly administered hydrogen peroxide as a source of oxygen for the extrapulmonary respiration in patient with severe influenza pneumonia. Ziegler (1941) has administrated the oxygen directly into the vein by the apparatus which he designed for the relief of the cyanosis in patient with heart disease. Feldman et al. (1966) reported that cats have been kept alive for periods up to one hour under ventilatory arrest by the infusion of hydrogen peroxide into the thoracic aorta. Shaw et al. (1967) observed that dogs administrated hydrogen peroxide by enema resulted the production of massive emboli in high concentrated hydrogen peroxide but not in low concentrated hydrogen peroxide. Of the effect of the continuous intravenous hydrogen peroxide infusion using concentration of 6,3, and 1.5% hydrogen peroxide with either 6% dextran or 5% glucose, Stern and Brennock (1967) have investigated there were massive emboli phenomena in the dogs during the course of the infusion but not in the cats. Through the experiments employing dilute hydrogen peroxide given by peritoneal or rectal perfusion, Urschel et al. (1966) suggested hydrogen peroxide of dilute solute can act as an adjunct for the cardiac resuscitation to be somewhat effective and possible future application of systemic anoxia. In the experiment of the intraperitoneal administration of 0.3% hydrogen peroxide in hypoxic rabbit as possible method of oxygen supply, Morgan et al. (1968) have investigated although initially hydrogen peroxide caused slight increase in arterial oxygen saturation, this increase was brief and followed by a marked fall in arterial oxygen saturation accompanied with hypotension, therefore, they suggested that the intraperitoneal administration of hydrogen peroxide for the purpose of extrapulmonary oxygenation was ineffective and hazardous. Britton Chance (1952) has reported in the experiment of respiring bacterial cell hydrogen peroxide may be separated to oxygen and water by catalase which participated in the control of hydrogen peroxide and perhaps in protection from ionizing radiation. Cohen (1963) has investigated peroxidase in erythrocyte was the important role of analysis of hydrogen peroxide and fundamental role in protecting erythrocyte against the toxic effects of hydrogen peroxide. They also suggested catalase activity is favored at high hydrogen peroxide concentration, while peroxidase activity is favored at low hydrogen peroxide concentration. White et al. (1966) have reported the catalase was present in human blood in both the plasma and red cell membrane, to which possibly catalase adhered. The amount of catalase in the blood varied greatly in different species, being high in man, low in the rat, very low in the dog and absent in the hen, therefore, when hydrogen peroxide solution was added to the blood of these latter animal, oxygenation did not occur; instead the blood turned dark brown color owing the formation of methemoglobin. Recently Yun (1931) investigated the effect of hydrogen peroxide enema with dogs which reported low concentrated hydrogen peroxide enema (less than 0 4%) by single dose resulted in the increased arterial oxygen tension without gas bubbles and high concentrated hydrogen peroxide enema (more than 0.5%) in the decreased arterial oxygen tension with gas bubbles. He also reported low concentrated hydrogen peroxide enema by repeated dose (less than 0.2%) resulted in the increased arterial oxygen tension without gas bubbles and high concentrated hydrogen peroxide enema (more than 0.25%) by repeated dose in the decreased arterial oxygen tension with gas bubbles. Paik (1969) studied histological change of liver tissue after administration of hydrogen peroxide and reported there was liver cell degeneration around branch with gas bubbles in high concentrated hydrogen peroxide but few weeks later he could not find out liver cell demage and gas bubbles. Kim (1971) investigated that single enema of human blood and hydrogen peroxide increased significantly arterial oxygen tension without gas bubbles. Lee (1972) reported that in the intravenous hydrogen peroxide administration there were marked elevation of methemoglobin in dogs but not in cat and rabbit, in hydrogen peroxide enema, slight elevation of methemoglobin in dogs, not in cat and rabbit, and in hydrogen peroxide enema with human blood, no change of methemoglobin in dogs, are noted. The present investigation is aimed at the studying the effect of 0.5% hydrogen peroxide enema, with human amniotic fluid, catalase, peroxidase, and human blood, and find out the change of arterial pH, pCO^^2 , pO^^2 . In the experiment adult dogs of either sex were used, and after 12 hours fasting state glycerine enema was done. Under the general anesthesia with seconal into the vein, dog's trachea was inserted by canula which connected the respirator for regulation of respiratory rate and volume. Respiration was performed with normal air and hypoxic air which contained 10% oxygen and 90% nitrogen. A long rectal tube was inserted and sutured at anus to prevent the leakage from infusion of hydrogen peroxide or other substances. It divided six groups by administrated subatances; 1) Control group which was administered saline solution only. (10 ml/kg). 2) Hydrogen peroxide group which was administered 0.5% hydrogen peroxide (10 ml/kg) only. 3) Amniotic group which was administered 0.5% hydrogen peroxide (10 ml/kg) and human amniotic fluid (1 ml/kg). 4) Catalase group which was adminstered 0.5% hydrogen peroxide (10 ml/kg) and catalase (6760 unit/cc). 5) Peroxidase group which was administered 0.5% hydrogen peroxide (10 ml/kg) and peroxidase (6050 unit/cc). 6) Human blood group which was administered 0.5% hydrogen peroxide (10 ml/kg) and human whole blood (1 m1/kg). Arterial blood was collected through femoral artery for the determination of pH, pCO^^2 pO^^2 , before enema and after enema (hypoxic state) at 15 min., 45 min., 75 min., in each group. The following results were obtained; 1) There were no significent change in the arterial pH, pCO^^2 before and after enema of all above groups. 2) In hydrogen peroxide group without any materials there were marked decreased pO^^2 in hypoxic state compared with normal air state and after enema more decreased arterial pO^^2 is noted as time passed. It is suggested methemoglobin is formed as the blood of dog which contained little catalase. 3) In amniotic group there were no change of pO^^2 before and after the enema of hydrogen peroxide. It is suggested amniotic fluid does nut contain catalase and peroxidase which separate hydrogen peroxide to water and oxygen. 4) In catalase, peroxidase and human blood group there were marked elevation of pO^^2 in hypoxic state. It is suggested hydrogen peroxide is separated into water and oxygen in the intestinal canal by catalase, peroxidase and blood which contains peroxidase and catalase. In this experiment catalase, peroxidase, human blood, were very effective to increase pO^^2 after hydrogen peroxide enema as an extrapulmonary respiration and were supposed to be a necessary materials to break down hydrogen peroxide into oxygen and water in the intestinal canal. human whole blood which contained a lot of catalase and peroxidase, is a good abundant source to be used as a catalysis in hydrogen peroxide enema for the purpose of extrapulmonary oxygenation.
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2. 학위논문 > 1. College of Medicine (의과대학) > 박사
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