The treatment of burn has been progressed great deal by mordernized fluid therapy and use of antibiotics. The nortality of patients from the burn, however, has not been finished(??) despite of the mordern therapy. This has made many researchers to
believe in existence of "burn toxin" and numerous papers concerning the burn toxin has been published in the past several decades. Through reviewing the papers it is suggested that there may be certain relation between the time following burn and the substances which are released from the burned skin.
In order to clarify the relationship as well as the existence of toxic substances and it's amount following burn, the animal experiments were carried out.
Diffusates were collected from subcutaneous air balloon of the back of rats after making 30 burn of the ballooned skin at 30 min. 1 hour, 2 hours, 4 hours, 12 hours, 24 hours, 48 hours and 72 hours intervals following burns. The amount of protein in each diffusates was measured. Each diffusate was then injected to the mouse intraperitoneally and the reaction of the mouse was carefully observed.
1. Average values of protein(s) in diffusate obtained from subcutaneous air balloon of burned skin of rats were 1.21 ± 0.069 mg/ml at 30 min., 0.8 ± 0.16 mg/ml at 1 hour, 1.04 ± 0.01 mg/ml at 2 hours, 0.87 ± 0.11 mg/ml at 4 hours, 1.0 ± 0.02 mg/ml at 6 hours, 0.85 ± 0.03 mg/ml at 12 hours, 2.39 ± 0.08 mg/ml at 24 hours, 2.92 ± 0.07 mg/ml at hours, and 4.06 ± 0.37 mg/ml at 72 hours.
2. All diffusates obtained from the burned skin of rats were lethal to the mice with it's toxic manifestation being from limitation of motion to the death of animal.
3. The toxicity of the diffusates obtained at 1 hour, 2 hours, 4 hours following burn was most violent and lethal to the mouse.