사람의 정상 난관 조직에서의 estrogen과 progesterone 수용체 농도

Abstract

[한글]

난관 평활근 수축은 estrogen과 progesterone에 의해 영향을 받는 것으로 알려져 왔으나, 현재까지도 난관 조직에서의 estrogen과 progesterone 수용체 농도에 관한 연구는 드물다.

본 연구에서는 난관의 해부학적 위치와 월경 주기에 따른 estrogen과 progesterone 수용체 농도를 조사하기 위하여, 정상 난관 조직을 가진 21명의 난관을 대상으로 난관 협부와 팽대부에서 estrogen과 progesterone 수용체 농도를 측정하였고, 이들을 월경 주기별로 분류하여 다음과 같은 결과를 얻었다.

1. 세포질내 estrogen 수용체 농도는 증식기의 난관 협부와 팽대부에서 각각 33.8±11.7 fmol/mg protein, 41.5±21.8 fmol/mg protein였고, 분비기에서는 각각 30.3±15.6 fmol/mg protein, 40.7±17.0 fmol/mg protein였으며 폐경후에서는 13.5±5.6 fmol/mg protein, 28.8±11.8 fmol/mg protein로서 협부보다 팽대부가, 분비기 또는 폐경후보다 증식기에서 높은 경향을 보였으며 특히 증식기 협부는 폐경후 협부에 비하여 유의하게 높았다(p<0.05).

2. 세포질내 progesterone수용체 농도는 증식기의 난관 협부와 팽대부애서 각각 265.1±243.8 fmol/mg protein, 413.4±301.5 fmol/mg protein였고, 분비기에서는 각각 69.9±74.0 fmol/mg protein. 23.4±7.0 fmol/mg protein였으며 폐경후에서는 23.4±7.0 fmol/mg protein, 69.4±26.7 fmol/mg protein로서 협부보다 팽대부가, 분비기 또는 폐경후보다 증식기에서 높은 경향을 보였으며 특히 증식기 협부는 폐경후 협부에 비하여, 증식기 팽대부는 분비기 팽대부에 비하여 유의하게 높았다 (P<0.05).

3. 핵내 estrogen수용체 농도는 증식기의 난관 협부와 팽대부에서 각각 12.0±13.6 fmol/mg protein, 12.8±10.0 fmol/mg protein였고, 분비기에서는 각각 8.6±7.2 fmol/mg protein, 10.5±4.8 fmol/mg protein였으며 폐경후에서는 7.7±3.5 fmol/mg protein, 8.7±4.8 fmol/mg protein 로서 협부보다 팽대부가, 분비기 또는 폐경후보다 증식기에서 높은 경향을 보였다.

4 . 핵내 progesterone수용체 농도는 증식기의 난관 협부와 팽대부에서 각각 213.6±98.8 fmol/mg protein, 154.6±134.2 fmol/mg protein였고, 분비기에서는 각각 105.9±41.4 fmol/mg protein, 110.5±78.6 fmol/mg protein였으며 폐경후에서는 367.3±331.3 fmol/

mg protein, 332.5±284.8 fmol/mg protein로서 협부보다 팽대부가, 분비기보다 증식기에서 높은 경향을 보였고 협부 분비기에 비해 협부 증식기에서 유의하게 높은 수용체 농도를 나타내었다.(P<0.05).

이상의 결과로 estrogen과 progesterone 수용체 농도는 난관의 팽대부에서, 월경 주기별로는 분비기나 폐경기에 비해 증식기에서 높은 경향을 보였으며, 이들 수용체의 정상 농도와 주기별 농도의 차이등은 난관의 분절운동과의 상관 관계 또는 난관 요인에 의한

불임등 이상 난관 운동의 요인을 평가하는데 기초자료로서 가치가 있으리라 사료된다.

[영문]

It is known that uterine tubal smooth muscle contraction is mainly controlled by estrogen & progesterone, but until recently, studies regarding estrogen and progesterone receptor concentrations present in fallopian tubes have been rare.

This study evaluated the estrogen and progesterone levels in the isthmic and ampullar portions of normal tubes frets 21 patient to investigate the estrogen and progesterone receptor concentrations according to different anatomical portion of

the fallopian tubes and also according to menstrual cycle and after menopause.

And obtained following results after analysing these data.

1. The mean estrogen receptor concentrations in the cytoplasm of isthmic and ampullar portions, during the proliferative phase were 33.8 ± 11.7 fmol/mg, 41.5 ± 21.8 fmol/mg protein respectively. 30.3 ± 15.6 fmol/mg, 40.7 ± 17.0 fmol/mg protein during the secretory phase, and 13.5 ± 5.6 fmol/mg, 28.8 ± 11.8 fmol/mg protein after menopause, which showed that the estrogen concentration in the ampullar portion was higher than in the isthmic portion, and also higher in the proliferative phase than in the secretory phase or a post-menopausal state. The estrogen concentration in the isthmic portion during the proliferative phase was significant1y higher than in the isthmic portion of the post-menopausal state (P<0.05).

2. The mean progesterone receptor concentrations in the cytoplasm of isthmic and ampullar portions during the proliferative phase were 265.1 ± 243.8 fmol/mg, 413.4± 301.5 fmol/mg protein respectively, 69.9 ± 74.0 fmol/mg, 23.4 ± 7.0 fmol/mg protein during the secretory phase, and 23.4 ± 7.0 fmol/mg, 69.4 ± 26.7 fmol/mg protein during the post-menopausal state, which showed that the progesterone concentration of the ampullar portion was higher than in the isthmic portion, and

also higher in the proliferative phase than in the secretory phase or the post-menopausal state. The estrogen concentration in the isthmic portion during the proliferative phase was significantly higher than in the ampullar portion during the secretory phase (P<0.05).

3. The mean estrogen receptor concentrations in the nucleus of isthmic and ampullar portions during the proliferative phase were 12.0 ± 13.6 fmol/mg, 12.8 ± 10.0 fmol/mg protein respectively, 8.6 ± 7.2 fmol/mg, 10.5 ± 4.8 fmol/mg protein during the secretory phase, and 7.7 ± 3.5 fmol/mg, 8.7 ± 4.8 fmol/mg protein after menopause, which showed that the estrogen receptor concentration in the ampullar portion was higher than in the isthmic portion, and also higher the proliferative phase than in the secretory phase or the post-menopausal state.

4. The mean progesterone receptor concentrations in the nucleus of isthmic and ampullar portions during the proliferative phase were 213.6 ± 98.8 fmol/mg. 154.6 ± 134.2 fmol/mg protein respectively, 105.9 ± 41.4 fmol/mg, 110.5 ± 78.6 fmol/mg

protein during the secretory phase, and 367.3 ± 331.3 fmol/mg, 332.5 ± 284.8 fmol/mg protein during the post-menopausal state, which showed that the concentration in the ampullar portion was higher than in the isthmic portion, and also higher in the proliferative phase than in the secretory phase or the post-menopausal state (P<0.05).

According to these results, the estrogen and progesterone receptor concentrations were higher in the ampullar portion than in the isthmic portion, and also these levels were higher during the proliferative phase than in the secretory phase or post-menopausal state.

We therefore conclude that evaluation of normal estrogen and progesterone receptor concentrations and of the difference according to different anatomical portions and different phase of the menstrual cycle could be benefit as basic data for studying the relationship between these concentration to and tubal segmental peristaltic movement, and for studying factors which cause abnormal uterine peristaltic movement in cases such as infertility caused by tubal factors.