Effects of ketamine on the isolated uterus of rabbits
[영문]Since McCarthy et al. (1965) demonstrated that ketamine produced a profound analgesia, preserved the protective pharyngeal reflex and elevated the arterial blood pressure in dogs, this anesthetic agent has been widely used in clinical
anesthesia. However, there has still been argument about its psychotomimetic effects such as postanesthetic deliruium and occasional hallucinations which appear to be commoner in adults than in children. On the other hand, numerous investigators (Chodoff and Stella, 1966; Moore et al., 1971; Little et al., 1972; Jawalekar et al., 1972; Galbert and Gardner, 1973; Akamatsu et al., 1974) reported that ketamine induced contraction of the uterus and recommend this drug as a suitable obstetric anesthetic agent.
The present study was undertaken to determine whether or not ketamine excites the myometrium and to elucidate the mechanism of its uterine action.
Female albino non-pregnant, pregnant and postpartum rabbits weighing approximately 2.0kg were employed in this experiment. At the end of ten days following bilateral oophorectory, the non-pregnant rabbits were injected intramusculary with estrogen(2000 I.U./kg) or progesterone (5mg/kg) daily for 4 days. An uterine strip, about 2.0cm in length, was carefully isolated from the
experimental rabbits and suspsended in a muscle chamber containing 100ml of Locke's soultion, maintained at a constant temperature of 38℃. It was aerated with 100% oxygen bubbing through the bathing fluid by means of a sintered glass plate at the bottom of the muscle chamber. The uterine strip was attached to a Grass force displacement transducer, and the motility and tonus were recorded on a Grass model 7 polygraph. After being washed several times with fresh Lock's solution, the uterine strip attained a constant motility and tonus. Ketamine then was added in concentrations ranging from 50 to 2000 r/dl to the muscle chamber.
1. Ketamine produced no appreciable effect on the spontaneous motility and tonus of the uterine strip isolated from the normal non-pregnant rabbits but in a few instances it produced an increase in the amplitude and frequency of contraction.
2. Ketamine showed a tendency to increase the amplitude and frequency of contraction of the uterine strips isolated from pregnant and postpartum rabbits.
3. Uterine strips isolated from bilaterally oophorectomized rabbits showed a very weak but regular spontaneous contraction. Ketamine produced neither inhibitory nor stimulatory effects on these uterine strips.
4. Ketamine produced no effect on the motility and tous of isolated uterine strips from rabbits teated with estrogen for 4 days following bilateral oophorectomy.
5. Ketamine prodcued, without exception, a marked stimulant action on the motility and tonus of the uterine strips isolated from rabbits treated with pregesterone following bilateral oophorectomy.
6. Pretreatment of the uterine strip from progesterone-treated rabbits with an adrenergic alpha blocking agent (dibenzyline) or adrenergic beta blocking agents(dichloroisoproterenol and propranolon) failed to alter the excitatory action of ketamine.
7. Atropin failed to alter the excitatory action of ketamine.
8. Oxytocin and ergonovine produced no effects on the excitatory action of ketamine on the isolated uterine strips from the progesterone-treated rabbits.
From the above results it may be concluded that ketamine exerted a stimulatory action on the uterus under the influence of progesterone. This progesterone-dependent uterine stimulatory action of ketamine is not concerned with adrenergic and cholinergic mechanisms but appears to be a direct action on the