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백서(白鼠)에 있어서 진동 stress로 인한 위점막 변화에 관한 실험 : 조직학적 변화와 cortisone 및 ACTH의 영향

Title
 백서(白鼠)에 있어서 진동 stress로 인한 위점막 변화에 관한 실험 : 조직학적 변화와 cortisone 및 ACTH의 영향 
Other Titles
 (The) effect of shaking stress on the lesions of the stomach of albino rats : The histological changes and the effect of cortisone and ACTH 
Issue Date
1968
Publisher
 연세대학교 대학원 
Description
의학과/박사
Abstract
[한글]
[영문] Singer(1913) first produced lesion in the rumen(forestomach) of rats by feeding with bread mixed with wood sawings. Hereafter, a number of workers produced lesion in the rumen by various methods. Sharpless(1937, 1940) also produced lesions in the rumen of the rats by feeding with low protein diet. He described that the early lesion was characterized by a greatly thickened squamous epithelial layer that the thickning was accompanied by papilloma formation at the surface and hypertrophy of the papillae at the base, that ulceration of the epithelium might occur and the ulcer was usually superficial and that metastases of the lesion had never been found. On the other hand, Shay et al.(1945) produced ulcers of forestomach in rats by ligation of the pylorus. The rat was fasted for 48 to 72 hours, and then pylorus was ligated for 16 to 18 hours. Later Robert and Nezamis(1964) produced the same ulcers of forestomach without ligation of pylorus but only by fasting of the rat for four days. Levrat and Lambert(1959) produced ulceration in the glandular portion by shaking stress and intermittent photic stimulation. But Lee(1964), Moon(1964) and Park(1967) produced lesions of the forestomach by shaking stress by without photic stimulation. They fed the rats on the diet of biologically low protein diet. Kim and Lambooy(1967) produced ulceration in the glandular portion by shaking stress but they fed the rats on riboflavin deficient died. The purpose of this experiment is to investigate the effect of ACTH and cortisone on the production of lesions in the rumen of rats fed on rice diet. The lesions of the forestomach was investigated in detail microscopically and esophagus was also investigated microscopically. Materials and Methods Apparently healthy male young albino rats were fed on rice diet from 10 to 15 days earlier from the beginning of the experiment. Then the rats were divided into two groups. To the experimental groups cortisone and ACTH was intraperitoneally injected everyday and other group was used as control given the same amount of normal saline solution. The rats were shaken 4 hours daily according to the method of Moon(1964). The rats were shaken with frequency of 180 per minute on the first day, and thereafter the frequency increased 20 per minute everyday until the frequency reached to 260 per minute. The shaking stress continued for 7 days. Then the rats were sacrificed and the stomach was examined macroscopically and histologically. Also the esophagus was examined microscopically. Result Observations of the Stomach 1. The experiment given 1.5mg of cortisone. a. Macroscopic findings: In the control groups, 4 of 8 rats produced the forestomach ulcerations and in the experimental groups, 3 of 8 rats. The slight edema was found around the ulceration accompanying cellular hypertrophy(Table 5). b. Histological findings: In the control groups, severe histological changes were found in the gastric mucosa: remarkable proliferation and cornification of the epithelial layer occurred around the ulcerations, and the inflammatory lamina propria was characterized with infiltration of neutrophile and eosinophile leucocytes. The lamina muscularis mucosae and submucosa just below the ulceration revealed extensive edematous infiltrations of the white blood cells. In the experimental groups, epithelial proliferation and cornification were not easily observed. The ulceration sites which filled with tissue exudates, were often found. NO severe edematous figures were observed in the lamina propria, but the infiltrations of the eosinophile and neutophile leucocytes occured as the control groups. The lamina mucsularis mucosae was similar to that of the control group. The submucosa also revealed weaker inflammatory changes than that of the control group. The 3 cases of esophageal ulcerations in this experimental group were found. 2. The experiment given 3.0mg of cortisone: a. Macroscopic findings: In the control groups, 6 of 8 rats produced the forestomach ulcerations, which diameters were about 3mm. It is characterized by 2 cases of colonied ulcerations formed of 7 or 8 and sever edematous, hypertrophied, and cornified figures were also found. b. Histological findings: In the control groups, changes were gernerally similar to those of the 1st experiment: epithelial layer accompanying remarkable cornification and cell proliferations, the lamina propria infiltrated with eosinophiles and neutrophiles. Besides these, dilations of the venules and blood capillaries were noticeable. The white cell infiltration of the lamina muscularis mucosae just below the ulceration site was similar to that of the lamina propria and the submucous layer also revealed the same vascular changes as the lamina propria. In the experimental groups, slight cornification and proliferation occurred in the epithelial layer and also scarce infiltration of the white cells occurred in the lamina propria. The lamina muscularis mucosse just below the ulceration site was similar to that the control group. 3. The experiment given 6.0mg of cortisone: a. Macroscopic findings: In the control groups, 3 of ulceration in 8 rats occurred. In the experimental groups, one of ulceration in 7 rats occurred except one expired case. No colonied ulcerations were observed. However, most of the ulcerations were large, remarkable and which boundaries were more than 3mm in diameter. The glandular ulcerations which has not been found before occurred in 2 cases of these experiments were observed, although their macroscopic changes were slight. b. Histological findings: In the control groups, the epithelial layer revealed slight cornification and proliferation. But the lamina propria revealed moderate degree of edematous changes, slight connective tissue proliferations, and the white cell infiltrations. Some congestion figures of the venules and blood capillaries were observed in the lamina propria. The white cell infiltrations also occurred in the lamina muscularis mucosae underlying the ulceration as the lamina propria. Severe infiltrations of the white cells were found in the submucosa and slight congestions of the vessels were also found. In the experimental groups, the ulceration changes were similar to those of the control groups. In this experiment one case of the esophageal ulceration occurred. 4. The experiment given 3.0 IU of ACTH: a. Macroscopic findings: In the control groups, 2 of the 7 rats revealed the ulcerations. In the experimental groups, no ulcerations were found. The changes by the gastric ulcer were slight. b. Histological findings: In the control groups, the epithelial layer revealed slight or moderate degree of cornification and proliferation accompanying inflammatory changes and infiltrations of a number of the neutrophiles and small amount of the eosinophiles. Moderate or sever edematous changes, infiltrations of many neutrophiles, small amount of eosinophiles and lymphocytes were found in the lamina propria. Remarkable dilation of the venules and congestions of the blood capillaries were also easily found. The lamina muscularis mucosae also revealed infiltration of the neutrophile leucocytes. Sever infiltrations of a number of the neutrophiles and small amount of the eosinophiles, and congestions of the capillaries and proliferation figures of the connective tissues occurred in the submucosa. In the experimental groups, no remarkable histological changes were found and all of the gastric layers were identified to be those of the normal. 5. The experiment given 6.0 IU pf ACTH: a. Macroscopic findings: In the control groups, the ulcerations occurred in 3 of the 7 rats. In the experimetntal groups, only one case occurred. The ulceration changes were more remarkable in the control group than in the experimental one. b. Histological findings: In the control groups, the epithelial layer with a ulceration site accompanied slight proliferation and cornification. Some infiltration figure of the neutrophile leucocytes was found around the gastric ulcerfilled withe substances to be considered as connective tissues. In the lamina propria, moderate edematous changes, and infiltrations of numbers of eosinophiles and small amount of the neutrophiles were observed. The lamina muscularis mucosae just below the ulceration site revealed slight degree of the white cell infiltrations. A number of eosinophiles leucocytes were infiltrated into the submucosa. In the experimental groups, all of histological findings were similar to those of the control groups, and which changes were very slight. Observations of the Esophagus. As the histological structures of the epithelium in the esophagus were similar to those of the forestomach, the similar observation method was applied also in the esophagus. In the esophagus, 4 cases of 54 experiments revealed the esophageal ulcerations and were histologically confirmed. Generally the ulcers found in the esophagus appeared only in the epithelial layers, and were characterized by hemorrhage and erosion. The epithelial layers around the esophageal ulcerations revealed slight proliferative and cornified changes. But the lamina propria around the ulcer and the submucosa just below the ulcer revealed little immflammatory changes as those of the forestomach ulcerations. Summary The pathological changes of the forestomach produced by shaking stress in albino rats fed on low protein diet and those rats pre-treated with cortisone or ACTH prior to shaking stress were investigated macroscopically and histologically. The following results were obtained in this study. 1. The lesions of the forestomach produced by shaking stress were accompanied by edema, hyperplasia, and cornification of the gastric mucosa. 2. The pathological changes of the forestomach observed in rats treated with cortisone or ACTH prior to shaking stress were less severe than those in rats pre-treated with only shaking stress. 3. Ulceration was also observed in the esophagus where inflammatory changes were slighter than those of the forestomach. Summarizing the above results, the lesions of the forestomach in albino rats fed on low protein diet were produced by shaking stress. It is likely that cortisone or ACTH act more or less as inhibiting agents to gastric ulceration of albino rats.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/115489
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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