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적출된 토끼와 자라심장에서의 Ca++Pool

 적출된 토끼와 자라심장에서의 Ca++Pool 
Other Titles
 Ca++ Pools in isolated rabbit and turtle heart 
Issue Date
 연세대학교 대학원 
[영문] From the study of movements of Ca**++ in frog cardiac muscle, Niedergerke (1963) postulated that Ca**++ necessary for the cardiac contraction is stored in a specific pool. Langer et al (1967) and DeCaro (1967) also found a close relationship between the change of Ca**++ flux kinetics and the change of contractile force. According to the studies of several investigators, Ca Ⅱ (Bailey and Dressel 1968) or phase Ⅰ and Ⅱ (Langer 1965, Langer et al 1967, 1971) in the Ca**++ washout curve was associated with cardiac contractility. This investigation was aimed to elucidate the anatomical region of the contractile active Ca**++ pool. At the same time, it was assumed in this study that Ca**++ in the sarcoplasmic reticulum represent one of the major intracolluar Ca**++ pool and cardiac contractility was also dependent on the intracellular Ca**++ concentration. Consequently, this experiment was performed at different temperatures to activate or inhibit the deactivating process of activated Ca**++ in the intracellular space to see if changes in the contractility decay curve existed at different temperatures. The isolated hearts of rabbits and turtles (Amyda maackii) were attached to the perfusion apparatus according to the method employed by bailey and Dressel (1968). The isolated hearts were initially perfused with a full ringer solution containing 2 mg/ml of inulin for 1hr, and then Ca**++ and inulin-free Ringer solution was perfused while the isometric tension was recorded and a serial sample of perfusion fluid dripping from the cardiac apex was collected for 10 sec. throughout experimental period. The above procedure was performed at 22℃, 30℃ and 38℃ on the rabbit heart and 10-15℃, 20℃, 30℃ and 35℃ on the turtle heart. After determination of Ca**++ and inulin concentration of the samples, the Ca**++, inulin washout curve and the contractile tension decay curve were analysed according to the method of Riggs (1963). The results were summarized as follows; 1. In the rabbit heart, there are 2 inulin compartments, 3 Ca**++ compartments and single exponential decay of contractile tension. In the turtle heart, there are 1-2 inulin compartments, 1-2 Ca**++ compartments and 1-2 phases of contractile tension decay. The fact that the inulin space was divided into 2 compartments in the washout carve in these hearts indicates the presence of heterogeneity in cardiac perfusion, i.e., over-perfused and under-perfused area. 2. Ca Ⅰ and CaⅡ in these hearts were found to have Ca**++ in the ECF compartments because their half times in the washout curves were far smaller than those of the inulin washout curves in the rabbit heart and similar to those of the inulin washout curves in the turtle heart. Ca Ⅱ in the rabbit heart may have originated from the intracellular Ca**++ store. But no Ca Ⅲ in the turtle heart was found. This may be due to the fact that the intracellular Ca**++ pool in the turtle heart was too small to detect using this experimental procedure since sarcoplasmic reticulumn in the turtle heart is poorly developed. 3. In the rabbit heart, there were no changes in the half time of Ca Ⅰ, Ca Ⅱ, , inulin Ⅰ and inulin Ⅱ at different temperatures, but the half time of Ca Ⅲ was significantly prolongea at lower temperatures, and the half time of the contractile tension decay tended to be prolonged at lower temperatures but this was not significant. In the turtle heart, there were no changes in the half time of Ca Ⅰ, Ca Ⅱ, inulin Ⅰ, inulin Ⅱ and phase Ⅰof the contractile tension decay at different temperatures, but the half time of phase Ⅱ of the contractile tension decay was significantly prolonged at lower temperatures. This finding indicates that intracellular Ca**++ in these hearts was also responsible particulary for meantaining the cardiac contractility at the lower temperatures. 4. The half times of contractile tension decay were shorter than those of Ca Ⅱ in the Ca**++ washout curves in both animal hearts. According to the above results it was shown that Ca**++ in ECF is primarily and Ca**++ in the intracellualr space is partially associated with the cardiac contractility.
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2. 학위논문 > 1. College of Medicine (의과대학) > 석사
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