Steroid (Cortisone acetate) 투여 및 Gamma선 조사한 한국산 다람쥐에 있어서 실험적 결핵증에 관한 연구

Abstract

[한글]

[영문]

The detection of the tubercle bacilli is absolutely necessary to make a diagnosis of tuberculosis. Recent reports state that the rate of detection of tubercle bacilli in the patient's sputum or urine is decreasing because of the abuse of anti-tuberculous drugs and the resistance developed in the experimental animals

such as mice and guinea pigs. (Marks. 1972).

Therefore the necessity for the development of a better diagnostic method has been mandatory. Recently, its has been reported that Korean chipmunks (Tamias sibiricus asiaticus, Gmelin)are highly susceptible to tubercle bacilli (Chang et

al, 1971). Also it has been known that steroid administration and whole body irraduatuin results in immunosuppression in experimental animals. The specific object of this study is to find a more accurate and prompt mehtod for the detection of tubercle bacilli in a patient's urine or sputum and to allpy the method clinically.

Hence the effect of cortisone and gamma-ray irradiation on experimental tuberculosis in Korean chipmumks has been studied.

Tubercle bacilli, H37 Rv, were inoculated into the chipmumks peritoneal cavity to induce tuberculosis. The Korean Chipmunks were divided into eight experimental groups of 10 animals each in the test groups and 5 in the controls.

Group Ⅰ. Simultaneously treated :

An injection with cortisone acetate 5mg.

was given daily after the bacilli inoculation.

Ⅱ. Pre-treated :

An injection with cortisone acetate 5mg.

was given daily for one week prior to bacilli inoculation.

Ⅲ. Post-treated :

An injection with cortisone acetate 5mg.

was given daily one week after bacilli inoculation.

Ⅳ. Whole body gamma-ray irradiation

Gamma-ray irradiation was given just before the bacilli inoculation.

Groups Ⅴ.Ⅵ.Ⅶ. and Ⅷ. were controls.

Every week during the experiment, 2 test and 1 control animals were weighed and sacrificed for observation of tuberculous changes in the peritoneal fluid, spleen, lung, liver and kidneys.

The results are briefly summarized as follows :

1. Compaired with tuberculous control, Group Ⅰ,Ⅱ. and Ⅲ. revealed a miled decrease in body weght until the 4th week of the experiment. In comtrast, Group Ⅳ. showed a marked decrease until the 18th day and thereafter none had survived.

2. In Group Ⅰ, tubercle bacilli were found in the peritoneal fluid, spleen, liver and lung on the 7th day of the experiment. There were very significant tuberculous changes when compaired with other groups.

3. Compaired with GroupⅠ, Group Ⅳ. showed less significant tuberculous change by pathologic examination and in the bacterial count Ridley index on 1st week of the experiment.

Thereafter, the disease process abruptly became severe.

4. These fingings suggest that cortisone administration for the purpose of immunosuppression in the tuberculous Korean chipmunks induces the disease to become more severe and spreads the tuberculosis extensively. So it is belived that if sputum or urine is inoculated into immunosuppressed animals, such as cortisone treated Korean chipmunks, the rate of detection of tubercleb acilli would be higher and more accurate.

Further effort is necessary for the clinical application of use of the immunosuppressed animals as a better diagnostic tool.