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Cortisone acetate를 투여한 백서태반의 형태학적 연구

Other Titles
 (The) effects of cortisone acetate on the rat placenta 
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[영문] Glucocorticoids, such as cortisone have been known to be teratogenic in mice and also induce reabsorption and abortion of the fetus in pregnant rabbits, rats, mice, and guinea pigs(Domm and Leroy, 1951; Leroy and Domm, 1951; DeCosta and Abelman, 1952; Robson and Sharaf, 1952; Lee and Ring, 1956; Ingallis and Curley, 1957). But, it is not known whether cortisone produces fetal malformation by direct action on the embryo or through some action on the pregnant female (Kalter, 1965). However the fact that fertility as well as uterine, placental, and fetal development is in several ways dependent upon maternal adrenocorticoid hormone production is now well documented (Amoroso, 1955). To date, only a few investigators have recorded cortisone-induced morphological changes in the placentas of experimental animals. The present study is an attempt to investigate the probable site of influence in the placenta by cortisone, that could be related to fetal death, reabsorption, using the light and electron microscopic examinations in albino rats. Materials and Methods Female albino rats weighing around 200 gms. were used for the experiment and mated. The pregnant females were divided into two groups as follows. Group Ⅰ: Normal control (35 pregnant rats) Group Ⅱ: Cortisone treated (35 pregnant rats) Each group was subdivided into seven groups. In the experimental group, daily dose of 5 mg of cortisone acetate per animal was given intramuscularly. The placentas were obtained on the 10th, 12th, 14th, 16th, 18th, 19th, and 20th day of the pregnancy in both experimental and control groups. For histologic examination the routine hematoxylin-eosin staining method was performed, and PAS staining method for mucopolysaccharide and glycogen, methyl-green pyronin staining method for RNA, and reticulum staining method for reticulum fiber was also applied. For the electron microscopic examination the tissue was fixed in 1% osmium tetraoxide in phosphate buffer of pH 7.4 for 2 hours, and embedded in Epon 812 after dehydration. Sections were made with a glass knife of 400 to 500 A in thickness and stained with uranyl acetate and lead hydroxide. Observations were made with Hitachi HU 11-E model electron microscope. The following results were made in observation of the placental changes of the pregnant rats treated with cortisone acetate by the light and electron microscopy. 1) The wet placental weights of cortisone-treated groups were decreased in comparison with that of the control group. 2) In cortisone-treated groups even though the morphologic changes of vitelline membrane and junctional zone of the placenta were not much different from those of the control group, the placental labyrinth showed early congestion and atrophy, with early degenerative changes of trophoblasts. 3) The positive periodic acid Schiff stained material in the trophoblasts of the rat placenta treated with cortisone was relatively decreased at the late gestational period. Meanwhile the methyl-green pyronin reaction of trophoblasts in those group showed decreased reaction of trophoblasts in early phase of placental formation. 4) The dilatation of rough endoplasmic reticulum and detachment of ribosomes in trophoblasts of early placental formation were found by electron microscopy. On the basis of the above findings the cortisone induces marked congestion of placental labyrinth, atrophy and degeneration of trophoblasts, decreased positive meterial of PAS, and interference of protein synthesis in the rat placenta. It is speculated that those changes might be related with the death, abortion and reabsorption of the fetus.
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2. 학위논문 > 1. College of Medicine (의과대학) > 박사
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